Mapping gustatory neuron types.

映射味觉神经元类型。

基本信息

批准号：
10569149
负责人：
Nirupa Chaudhari
金额：
$ 3.95万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10569149
关键词：
Address Afferent Neurons Anatomy Antibodies Bioinformatics Brain Brain Stem Citric Acid Code Color Data Dextrans Duct (organ) structure Ear FOS gene Foliate Papilla Fungiform Papilla Genes Genetic Markers Genetic Recombination Heterogeneity Hybrids Image Immunofluorescence Immunologic Incisor Individual Label Modality Molecular Mouse Strains Mus Nerve Neural Pathways Neurons Nucleus solitarius Nutrient Oral Pathway interactions Peripheral Population Process Property Receptor Cell Reporting Research Resolution Role Sensory Sensory Ganglia Signal Transduction Sodium Glutamate Soft Palate Spinal Ganglia Stimulus Structure Structure of geniculate ganglion Sucrose Tactile Taste Bud Cell Taste Buds Taste Perception Techniques Testing Texture Transgenic Mice base cell type dorsal horn experimental study in vivo insight mechanical stimulus molecular marker neuronal circuitry novel receptive field response segregation single-cell RNA sequencing sugar transcriptome

项目摘要

PROJECT SUMMARY The afferent sensory neurons that innervate taste buds have eluded definition at the molecular level until very recently. Specifically, it has been unclear if separate classes of neurons innervate the well-established receptor cell types in the taste bud. It has also been unclear whether there are dedicated neuronal circuits that connect peripheral receptor cells, afferent sensory neurons and the first relay neurons in the Nucleus of the Solitary Tract in the brainstem. Progress has been impeded by the lack of molecular markers to distinguish gustatory neuronal cell types. We have recently completed a study on single-cell RNAseq of geniculate ganglion neurons. Our detailed bioinformatics analyses, which are publicly accessible, revealed neuronal subtypes based on deep separations in their transcriptome profiles. Our study also yielded markers which permit us to distinguish the classes of gustatory neurons. We propose to examine the three main classes, T1, T2 and T3, in several ways. We will assess whether each class of neurons associates with taste buds in a given receptive field, or with particular taste bud cells types. We will also examine the central projections of neurons of each class for insights into their potential roles in gustation. Second, we will evaluate whether the gustatory neuron types preferentially convey information on particular taste qualities. And finally, we will examine the significance of the novel hybrid gustatory-mechanosensory neuron type that we recently reported, using in vivo confocal Ca2+ imaging in transgenic mice expressing the Ca2+ indicator, GCaMP. Together, these analyses will be the first to assess the extent of heterogeneity in the peripheral and central connectivity and functional properties of defined subtypes of gustatory neurons.

项目摘要支配味蕾的传入感官神经元在分子上避免了定义直到最近。具体而言，目前尚不清楚是否单独支配味蕾中良好的受体细胞类型。还不清楚是否有连接外围受体细胞的专用神经元电路，传入感觉神经元和脑干孤立核细胞核中的第一个继电器神经元。缺乏分子标记来区分味觉神经元，阻碍了进展细胞类型。我们最近完成了一项关于Geniculate Ganglion单细胞RNASEQ的研究神经元。我们详细的生物信息学分析揭示了神经元基于其转录组曲线的深度分离的亚型。我们的研究也产生使我们能够区分味觉神经元的标记。我们建议通过多种方式检查三个主要类T1，T2和T3。我们将评估每个是否一类神经元与给定的接收领域或特定味蕾的味蕾相关联细胞类型。我们还将检查每个班级的神经元的中心预测以了解见解进入他们在阵阵中的潜在作用。其次，我们将评估味觉神经元是否类型优先传达有关特定味道的信息。最后，我们将检查我们最近的新型混合味觉机械性神经元类型的重要性报道，在表达Ca2+指示器的转基因小鼠中使用体内共聚焦Ca2+成像， GCAMP。总之，这些分析将是第一个评估异质性程度的分析味觉定义的亚型的外围和中央连通性以及功能特性神经元。