Imaging Molecules and Circuits in Peripheral Taste Pathways

外周味觉通路中的分子和电路成像

基本信息

批准号：
10020539
负责人：
Nirupa Chaudhari
金额：
$ 2.73万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-12-01 至 2020-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10020539
关键词：
Acids Afferent Neurons Animals Brain Calcium Cells Censuses Code Data Desire for food Esthesia Fatty acid glycerol esters Food Functional Imaging Ganglia Gene Expression Profile Genetic Transcription Human Hyperphagia Image Immunofluorescence Immunologic Individual Knowledge Label Laboratories Literature Maps Methods Modeling Molecular Profiling Mus Nerve Neurons Neurotransmitters Oral Oral cavity Pathway interactions Pattern Peripheral Preparation Proteins Publishing Reporter Resolution Reverse Transcriptase Polymerase Chain Reaction Rewards Salts Sensory Sensory Ganglia Serotonergic System Serotonin Signal Transduction Sodium Chloride Specialist Stimulus Structure of geniculate ganglion Taste Bud Cell Taste Buds Taste Perception Testing Transgenic Mice Transgenic Organisms Validation candidate marker chorda tympani design food consumption ganglion cell hindbrain individual response innovation molecular imaging molecular marker novel novel strategies public health relevance receptor red fluorescent protein response serotonin receptor sugar taste stimuli temporal measurement transcriptome sequencing transmission process

项目摘要

DESCRIPTION (provided by applicant): We have developed an innovative method to record taste-evoked activity in gustatory afferent neurons with good cellular and temporal resolution. The method uses confocal functional calcium imaging and transgenic mice that express GCaMP3 in sensory neurons. By carefully exposing geniculate ganglia in living, anesthetized GCaMP3-mice and applying taste stimuli to the oral cavity, we can now record robust and reliable responses to discern the principles behind the transmission of gustatory evoked signals from taste buds to the hindbrain. These data will considerably extend single unit electrical recordings from the chorda tympani and greater superficial petrosal nerves or from geniculate ganglion cells. We are able to recording simultaneously from large ensembles of neurons. We have also designed a powerful strategy to relate the functional response profiles of individual geniculate ganglion neurons to their patterns of gene expression and establish robust molecular markers for separate functional classes of neurons. In a concerted effort from two well-established laboratories, we now propose a multi-PI project to exploit this novel preparation and to answer key questions regarding taste. Our new approach will tremendously expand our knowledge of peripheral sensory processing in taste. Our preliminary data demonstrate the feasibility of all 4 tightly-focused Specific Aims: Aim 1: How do gustatory sensory afferent cells respond to sweet, salty, bitter, sour, umami tastes and fats? Aim 2: Do mixtures of taste stimuli enhance responses from gustatory sensory afferent neurons? Aim 3: Do ganglion neurons that express certain transmitter receptors innervate specific taste cells? Aim 4: Are there dedicated neurons that detect each taste stimulus, and if so, can specific molecular markers be identified that associate with gustatory afferent neuron responses for each taste quality?

描述（由申请人提供）：我们开发了一种创新的方法来记录具有良好细胞和时间分辨率的味觉传入神经元中的味觉引起的活动。该方法使用在感觉神经元中表达GCAMP3的共聚焦功能钙成像和转基因小鼠。通过仔细暴露在生活中的统一神经节，麻醉的GCAMP3小鼠并将口味刺激施加到口腔中，我们现在可以记录强大而可靠的响应，以辨别味觉诱发信号从味蕾传播到后脑的原理。这些数据将大大扩展来自Chorda Tympani和更大的浅表性质神经或Genication Ganglion细胞的单个单位电记录。我们能够同时记录大型神经元的集合。我们还设计了一种强大的策略，以将单个基因神经神经元的功能响应谱与它们的基因表达模式相关联，并为单独的神经元功能类别建立强大的分子标记。在两个完善的实验室的一致努力中，我们现在提出了一个多PI的项目，以利用这项小说的准备并回答有关口味的关键问题。我们的新方法将极大地扩展我们对口味外周感官处理的了解。我们的初步数据证明了所有4种紧密中心的特定目的的可行性：AIM 1：味觉感觉传入细胞如何应对甜，咸，苦，酸，酸，鲜味和脂肪的味道和脂肪？目标2：味觉刺激的混合物是否可以增强味觉传入神经元的反应？目标3：表达某些发射器受体的神经神经元是否支配特定的味觉细胞？ AIM 4：是否有专门的神经元检测到每种味道刺激，如果是，是否可以确定特定的分子标记物可以与每种味道质量相关的味觉传递神经元反应？