Proteomics Core

蛋白质组学核心

• 批准号: 10458588
• 负责人: Kevin George Hicks
• 金额: $ 4.51万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-05 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10458588
• 关键词: Area; Bioinformatics; Biological; Biological Assay; Blood; Cells; Cellular biology; Chemical Structure; Chemicals; Communities; Computer software; Data; Dialysis procedure; Disease; Disease model; Equilibrium; Erythrocytes; Event; Foundations; Goals; Health; Hematological Disease; Hematology; Hematopoiesis; Heme; Heme Iron; Homeostasis; International; Iron; Libraries; Mass Spectrum Analysis; Mediating; Metabolic Pathway; Metabolism; Metabolite Interaction; Methods; Modification; Non-Malignant; Output; Peptides; Post-Translational Protein Processing; Preparation; Process; Progress Reports; Proteins; Proteome; Proteomics; Regulation; Research; Sampling; Services; Signal Pathway; Signal Transduction; Structure; Surface; Techniques; Technology; Therapeutic Intervention; Tissues; Universities; Utah; base; functional group; innovation; insight; interest; member; metabolome; metabolomics; molecular hematology; novel; novel strategies; outreach; protein complex; protein function; protein metabolite; protein protein interaction; service member; targeted treatment

PROJECT SUMMARY The goal of the Protein-Metabolite Interactomics Core (PMIC) is to advance the understanding of protein- protein and protein-metabolite interactions in relation to iron, heme, hematopoiesis and red cell biology. We have developed a highly innovative approach to identify protein-metabolite interactions that regulate protein function. This approach is termed Mass spectrometry Integrated with equilibrium dialysis for the Discovery of Allostery Systematically or MIDAS, which utilizes purified proteins and a metabolite library to discover interactions between any soluble protein of interest and components of a defined metabolite library. We have validated our approach and shown that this platform, based on equilibrium dialysis, detects known and novel metabolite-protein interactions with very high sensitivity, independent of specific assays of protein function. This novel approach is able to discover unappreciated interactions that result in regulation of protein function. The CIHD will utilize the expertise provided by the existing U0fU Proteomics Core to provide a unique approach to discovery in the area of iron and heme homeostasis focused on combating nonmalignant hematologic diseases.

项目摘要 蛋白质量代谢物相互作用组核心（PMIC）的目标是促进对蛋白质的理解 与铁，血红素，造血和红细胞生物学有关的蛋白质和蛋白质 - 替代物相互作用。我们 已经开发了一种高度创新的方法来鉴定调节蛋白质的蛋白质代谢物相互作用 功能。这种方法称为与平衡透析集成的质谱法以发现 变构系统或MIDAS，它利用纯化的蛋白质和代谢物库来发现 任何感兴趣的可溶性蛋白质与定义的代谢产物文库的成分之间的相互作用。我们有 验证了我们的方法，并表明该平台基于均衡透析，检测已知和新颖的平台 具有非常高灵敏度的代谢产物蛋白相互作用，与蛋白质功能的特定测定无关。 这种新颖的方法能够发现导致蛋白质功能调节的不欣赏的相互作用。 CIHD将利用现有的U0FU蛋白质组学核心提供的专业知识来提供独特的 铁和血红素稳态领域的发现方法着重于对抗非恶性 血液学疾病。