Proteomics Core

蛋白质组学核心

基本信息

批准号：
10676106
负责人：
Kevin George Hicks
金额：
$ 4.51万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-05 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10676106
关键词：
Acceleration Area Bioinformatics Biological Biological Assay Blood Cells Cellular biology Chemical Structure Chemicals Communities Computer software Data Dialysis procedure Disease Disease model Education and Outreach Equilibrium Erythrocytes Event Foundations Goals Health Hematological Disease Hematology Hematopoiesis Heme Homeostasis International Iron Libraries Mass Spectrum Analysis Mediating Metabolic Pathway Metabolism Metabolite Interaction Methods Modification Non-Malignant Output Peptides Post-Translational Protein Processing Preparation Process Progress Reports Proteins Proteome Proteomics Regulation Research Sampling Services Signal Pathway Signal Transduction Structure Surface Techniques Technology Therapeutic Intervention Tissues Universities Utah functional group innovation insight interest member metabolome metabolomics molecular hematology novel novel strategies protein complex protein function protein metabolite protein protein interaction protein purification service member targeted treatment

项目摘要

PROJECT SUMMARY The goal of the Protein-Metabolite Interactomics Core (PMIC) is to advance the understanding of protein- protein and protein-metabolite interactions in relation to iron, heme, hematopoiesis and red cell biology. We have developed a highly innovative approach to identify protein-metabolite interactions that regulate protein function. This approach is termed Mass spectrometry Integrated with equilibrium dialysis for the Discovery of Allostery Systematically or MIDAS, which utilizes purified proteins and a metabolite library to discover interactions between any soluble protein of interest and components of a defined metabolite library. We have validated our approach and shown that this platform, based on equilibrium dialysis, detects known and novel metabolite-protein interactions with very high sensitivity, independent of specific assays of protein function. This novel approach is able to discover unappreciated interactions that result in regulation of protein function. The CIHD will utilize the expertise provided by the existing U0fU Proteomics Core to provide a unique approach to discovery in the area of iron and heme homeostasis focused on combating nonmalignant hematologic diseases.

项目概要蛋白质-代谢物相互作用组学核心（PMIC）的目标是促进对蛋白质-代谢物相互作用的理解与铁、血红素、造血和红细胞生物学相关的蛋白质和蛋白质代谢相互作用。我们开发了一种高度创新的方法来识别调节蛋白质的蛋白质代谢相互作用功能。这种方法被称为质谱法与平衡透析相结合，用于发现系统变构或 MIDAS，利用纯化的蛋白质和代谢物库来发现任何感兴趣的可溶性蛋白质与定义的代谢物库的成分之间的相互作用。我们有验证了我们的方法，并表明该平台基于平衡透析，可以检测已知和新颖的代谢物-蛋白质相互作用具有非常高的灵敏度，独立于蛋白质功能的特定测定。这种新颖的方法能够发现导致蛋白质功能调节的未受重视的相互作用。 CIHD 将利用现有 U0fU 蛋白质组学核心提供的专业知识来提供独特的铁和血红素稳态领域的发现方法侧重于对抗非恶性血液系统疾病。