Zeiss Lightsheet 7

蔡司 Lightsheet 7

• 批准号: 10430494
• 负责人: MICHAEL W. JENKINS
• 金额: $ 59.72万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10430494
• 关键词: 3-Dimensional; Area; Awareness; Brain region; Cardiology; Clinical; Collaborations; Communities; Confocal Microscopy; Core Facility; Developmental Biology; Embryonic Heart; Ensure; Fostering; Funding; Future; Histopathology; Image; Infrastructure; Institution; Investments; Light; Microfluidics; Microscope; Minor; Molecular; Neurology; Otolaryngology; Photobleaching; Phototoxicity; Research; Research Personnel; Resolution; Resources; Sampling; System; Time; Tissues; United States National Institutes of Health; Universities; base; clinical application; imaging system; instrument; instrumentation; light microscopy; medical schools; microscopic imaging; operation; programs; prostate biopsy; success; temporal measurement; virology; ward

Project Summary The School of Medicine’s Light Microscopy Imaging Core (SOM-LMIC) at Case Western Reserve University requests funding for a Zeiss Lightsheet 7 microscope, which would have an enormous benefit to our active research community. Currently, the Cleveland area does not have a commercial light-sheet microscope (LSM) available to its researchers. This is particularly surprising considering the excitement and increased use of LSM. Obtaining this imaging system would allow us to continue to provide state-of-the-art imaging to our expanding user base. The Zeiss Lightsheet 7 provides several advantages that our current instrumentation cannot provide, allowing our current users to ask new questions as well as attracting new projects we currently cannot support. First, the system enables one to acquire cellular resolution over sizeable fields of view. Many of our users have struggled to assess the spatial extent of molecular changes over larger regions (e.g., entire brain region, throughout embryonic heart, prostate biopsy, etc.). Second, the Zeiss Lightsheet 7 has much higher acquisition rates than standard confocal microscopy. Because an entire sheet is collected in comparison to point by point for confocal, the acquisition rates are much higher. This will benefit all of our users by enabling them to either save time, realistically image larger samples for a study, or provide higher temporal resolution for live imaging. Third, several of our users have problems with photobleaching or phototoxicity (e.g., live imaging applications). The Zeiss Lightsheet 7 only illuminates tissue that is being imaged which greatly reduces the required light levels and photobleaching/phototoxicity compared to confocal microscopy. The Zeiss Lightsheet 7 will ensure the continued success of many NIH-funded programs at CWRU and nearby institutions. For this proposal, we have identified 10 major and 4 minor users. This imaging system will allow our users to make contributions to a diverse number of fields including developmental biology (Atit, Bearer, Jenkins, Ward), 3D histopathology (Gupta, Madabhushi), virology (Levine), otolaryngology (McDermott), subcellular dynamics (Mears, Kisley), cardiology (Rollins), neurology (Tesar, Pieper), and clinical applications of microfluidics (Gurkan). We anticipate that this instrument will foster collaborations within our user groups and that other users will benefit from this resource especially after they become aware of its potential. Without the Zeiss Lightsheet 7 many of the projects described in this proposal will not be possible. As one of Case Western Reserve University’s 11 Institutional Core Facilities, significant investment into administration and infrastructure has been made to support our daily operations. To support the long-term operation of this instrument, the University has agreed to further contribute nearly 130k. The requested Zeiss Lightsheet 7 is essential to the needs of our evolving research, clinical and translational programs and will expand the range of viable projects in the future.

项目概要 凯斯西储大学医学院的光学显微镜成像核心 (SOM-LMIC) 请求资助蔡司 Lightsheet 7 显微镜，这将对我们的活跃 目前，克利夫兰地区没有商用光片显微镜 (LSM)。 考虑到 LSM 的令人兴奋和使用的增加，这一点尤其令人惊讶。 获得该成像系统将使我们能够继续为我们不断扩大的业务提供最先进的成像 Zeiss Lightsheet 7 提供了我们当前仪器无法提供的多项优势， 允许我们当前的用户提出新问题并吸引我们目前无法支持的新项目。 首先，该系统使人们能够在相当大的视野范围内获得细胞分辨率。 努力评估较大区域（例如整个大脑区域， 其次，Zeiss Lightsheet 7 的采集率更高。 速率高于标准共焦显微镜，因为是逐点收集整张纸。 对于共焦，采集率要高得多，这将使我们所有的用户受益，使他们能够执行以下任一操作。 节省时间，为研究提供真实的更大样本成像，或为实时成像提供更高的时间分辨率。 第三，我们的一些用户存在光漂白或光毒性问题（例如实时成像应用）。 Zeiss Lightsheet 7 仅照亮正在成像的组织，这大大降低了所需的光照水平 与共焦显微镜相比，蔡司 Lightsheet 7 的光漂白/光毒性将确保 CWRU 和附近机构的许多 NIH 资助项目持续取得成功 对于这项提案，我们有。 确定了 10 个主要用户和 4 个次要用户。该成像系统将使我们的用户能够为多元化做出贡献。 许多领域，包括发育生物学（Atit、Bearer、Jenkins、Ward）、3D 组织病理学（Gupta、 Madabhushi）、病毒学（Levine）、耳鼻喉科（McDermott）、亚细胞动力学（Mears、Kisley）、心脏病学 （Rollins）、神经学（Tesar、Pieper）和微流体的临床应用（Gurkan）我们预计这一点。 该工具将促进我们用户组内的合作，其他用户也将从该资源中受益 尤其是在他们意识到其潜力之后，许多描述的项目都没有使用 Zeiss Lightsheet 7。 作为凯斯西储大学的 11 个机构核心设施之一，这是不可能的。 我们对管理和基础设施进行了大量投资，以支持我们的日常运营。 为了支持该仪器的长期运行，大学已同意进一步捐款近130k。 所要求的 Zeiss Lightsheet 7 对于我们不断发展的研究、临床和转化的需求至关重要 计划，并将扩大未来可行项目的范围。