National Center for Quantitative Biology of Complex Systems

国家复杂系统定量生物学中心

• 批准号: 10426381
• 负责人: JOSHUA J COON
• 金额: $ 125.16万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-05 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10426381
• 关键词: Achievement; Alzheimer' s Disease; Automobile Driving; Biological; Biology; Biomedical Research; Biomedical Technology; Biotechnology; Cell physiology; Collaborations; Communities; Complex; Computer software; Data; Development; Diabetes Mellitus; Disease; Ensure; Evolution; Gene Expression; Genes; Genome; Genomics; Glean; Goals; Heart Diseases; Human; Internet; Knowledge; Laboratories; Large-Scale Sequencing; Legal patent; Lipids; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Metabolism; Mission; Molecular; Nucleic Acids; Pathogenesis; Physiology; Post-Translational Protein Processing; Process; Proteins; Proteome; Proxy; Publications; RNA; Regulation; Reporter; Research Personnel; Research Project Grants; Resources; Role; Scientist; Shoulder; Speed; System; Technology; Technology Transfer; Testing; Training; Training Programs; Transcript; Visit; Work; biological systems; community engagement; design; holistic approach; innovation; insight; interest; lipidome; material transfer agreement; metabolome; multiple omics; new technology; novel; success; tool; transcriptome

PROJECT SUMMARY The advent of genome technologies for interrogating gene expression has irreversibly changed the scale at which scientists investigate biological problems. More specifically, large-scale gene expression sequencing technologies have allowed us to glean insights into biology and disease pathogenesis at unprecedented pace. However, RNA levels are only one piece of a highly complex biological puzzle: genes are the starting point of the cellular regulatory process, while metabolites are often the end products and the ultimate biological effector molecules. There are many layers of regulation post-expression that fine-tune the biological system in the path from gene to protein to metabolite. Recognizing the central role of proteins and their post-translational modifications (PTMs) in this process, the National Center for Quantitative Biology of Complex Systems was founded to provide large-scale quantitative data for these molecules. Our Biotechnology Research Resource aims to accelerate the pace, depth, and accuracy of quantifying the proteome, metabolome, and lipidome. Driven by the needs of biomedical researchers, our mission is to develop technologies that provide rapid access to the most comprehensive and accurate reporters of the biological state. Specifically we will (1) extend and ultimately culminate our work to enable comprehensive biomolecule characterization; (2) develop and conclude our work enabling highly multiplexed proteome quantification and (3) break ground on the development of the novel chromatographic and mass spectrometry platform for wholly integrated multi-omic analysis. We shall develop these technologies in the context of several high impact driving biomedical research projects that require the new technological advancements for success and that can serve as technology testbeds. These driving projects comprise two central themes. First, responding to the demands of biomedical researchers to explore the roles of emergent and yet poorly understood biomolecules and their PTMs, we will target projects concerned with new, metabolite-derived post-translational modifications. Second, we have come to appreciate that in addition to protein measurements our collaborators require new technologies to identify and quantify metabolites and lipids to fully understand their biological systems of interest and we will work with a host of projects that allow us to test and refine technologies for the large-scale systems and multi-omics analyses to explore physiology and metabolism. Finally, we propose a multi-faceted approach to dissemination, training, and collaboration, with the ultimate aim of ensuring the sustained impact of our technology. All of our technologies are designed to be sustainable, and we have created both systematic and informal mechanisms to deliver our knowledge and innovations to the scientific community.

项目摘要 基因组技术用于审查基因表达的出现，不可逆转地改变了量表 科学家研究了生物学问题。更具体地，大规模基因表达测序 技术使我们能够以前所未有的速度了解生物学和疾病发病机理的见解。 但是，RNA水平只是一个高度复杂的生物学难题的一个：基因是 细胞调节过程，而代谢产物通常是最终产物和最终的生物效应子 分子。表达后有许多调节层，可以在路径中微调生物系统 从基因到蛋白质再到代谢产物。认识蛋白质及其翻译后的核心作用 在此过程中，修改（PTM）是复杂系统的国家定量生物学中心是 建立的是为这些分子提供大规模的定量数据。我们的生物技术研究资源 旨在加快量化蛋白质组，代谢组和脂肪姆的速度，深度和准确性。驱动 根据生物医学研究人员的需求，我们的使命是开发可快速访问该技术的技术 生物状态的最全面，最准确的记者。具体而言，我们将（1）扩展并最终 最终使我们的工作以实现全面的生物分子表征； （2）发展并结束我们的工作 实现高度多路复用的蛋白质组定量和（3）新颖的开发中的破裂 用于完全集成多摩变分析的色谱和质谱平台。 我们将在几种高影响力推动生物医学研究项目的背景下开发这些技术 这需要新的技术进步才能取得成功，并且可以用作技术测试台。这些 驾驶项目包括两个中心主题。首先，回应生物医学研究人员对 探索新兴且知之甚少的生物分子及其PTM的作用，我们将针对项目 与新的，代谢物的翻译后修饰有关。第二，我们开始欣赏 除了蛋白质测量外，我们的合作者还需要新技术来识别和量化 代谢物和脂质，以充分了解其感兴趣的生物系统，我们将与许多 使我们能够测试和完善大规模系统和多摩学分析的项目 探索生理和代谢。最后，我们提出了一种多方面的传播，培训和 协作，最终目的是确保我们技术的持续影响。我们所有的技术 旨在可持续 对科学界的知识和创新。