Biosynthesis of antifungal nucleoside antibiotics-Undergrad research supplement

抗真菌核苷抗生素的生物合成-本科生研究补充

基本信息

批准号：
10393814
负责人：
Kenichi Yokoyama
金额：
$ 0.97万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10393814
关键词：
3-hydroxybutanal Acids Actinobacteria class Administrative Supplement Amides Amination Amino Acids Anabolism Analytical Chemistry Antibiotics Antifungal Agents Antifungal Antibiotics Area Biochemistry Biomedical Research COVID-19 Carbohydrates Cell Wall Cellular Structures Characteristics Chemicals Chemistry Chitin Clinical Communicable Diseases Coupling Development Enzymatic Biochemistry Enzymes Exhibits Foundations Fungal Components Genome Goals Group Meetings Growth High Pressure Liquid Chromatography Human Immunocompromised Host Investigation Kinetics Knowledge Learning Ligase Ligation Mentors Mining Mycoses NF-kappaB-inducing kinase Natural Products Nucleosides Oxygenases Parents Pathway interactions Pharmaceutical Chemistry Phosphorylation Physical condensation Policies Polysaccharides Postdoctoral Fellow Preparation Public Health Publications Reaction Research Research Project Grants Structure Students Substrate Specificity Synthesis Chemistry Techniques Testing Toxic effect Training Travel Work alpha ketoglutarate analog career chemical synthesis combat experience experimental study fungus in vivo interest nikkomycin novel pathogenic fungus summer research undergraduate research undergraduate research experience undergraduate student

项目摘要

Project Summary/Abstract This administrative supplement will support a 10-week long research project for an undergraduate student. The parent R01 projects aims to understand the biosynthesis of peptidyl nucleoside (PN) antifungal antibiotics that are active against multiple pathogenic fungi and to discover novel antifungal agents using this knowledge. The proposed summer research project focuses on the part of Aim 3 of the parent R01 involving the NikS amide ligase enzyme. Specifically, the potential for use of NikS in the chemoenzymatic preparation of PNs will be investigated. It is anticipated that the undergraduate student will purify NikS, chemically synthesize ~10 amino acid substrate analogs of NikS and use the resulting compounds to test the substrate specificity and the potential usage of NikS for chemoenzymatic preparation of unnatural PNs. This project will provide the student with research experience in synthetic chemistry, bioorganic chemistry and enzymology as well as general knowledge about antifungal antibiotics discovery, biosynthesis and development. Thus, the project will significantly extend the research experience of the undergraduate student towards her goal of pursuing a biomedical research career.

项目摘要/摘要这种行政补充剂将为一名本科生提供为期10周的研究项目。这母体R01项目旨在了解肽基核苷（PN）抗真菌抗生素的生物合成，对多种致病性真菌具有活性，并使用这些知识发现新型抗真菌剂。这拟议的夏季研究项目重点介绍涉及NIKS AMIDE的父母R01的AIM 3的一部分连接酶酶。具体而言，在PN的化学酶制剂中使用NIK的潜力将是调查。预计本科生将净化NIK，化学合成〜10氨基 NIK的酸基板类似物，并使用所得化合物测试底物特异性和电势使用NIKS进行非自然PN的化学酶制剂。该项目将为学生提供合成化学，生物有机化学和酶学以及一般知识的研究经验关于抗真菌抗生素的发现，生物合成和发育。因此，该项目将大大扩展本科生的研究经验是她追求生物医学研究的目标职业。