Protein purification instrumentation in support of single molecule studies of genome integrity

支持基因组完整性单分子研究的蛋白质纯化仪器

• 批准号: 10386035
• 负责人: Eric C Greene
• 金额: $ 9.34万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10386035
• 关键词: Address; Biological; Cancer Biology; Cells; Chromosomal Rearrangement; Complex; DNA; DNA Double Strand Break; Development; Equipment; Event; Goals; Individual; Lead; Malignant Neoplasms; Methodology; Microscopy; Molecular; Nucleic Acids; Optics; Positioning Attribute; Proteins; Recombinant Proteins; Research; System; Technology; Time; Visualization; cancer genome; genome integrity; insight; instrumentation; interest; novel; prevent; protein purification; repaired; single molecule; success

My group has a long–standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real–time by optical microscopy. This experimental platform is applicable to many types of protein–nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity. Our use of this novel single molecule methodology is dependent upon access to highly purified recombinant proteins. This Supplemental Equipment request will allow us to replace our outdated protein purification system so that we can continue moving forward on our research efforts into understanding the mechanisms of genome integrity.

我的团队长期以来对了解分子机制感兴趣 为癌症生物学和基因组完整性做出贡献，提供有关机制的新见解。 为了保证基因组的完整性，我们率先开发了 DNA 帘幕，这使得 通过光学实时直接可视化数百至数千个单个分子 该实验平台适用于多种类型的蛋白质-核酸。 相互作用，使我们能够解决无法解决的复杂生物学问题 我们在这项技术上的成功是通过传统方法解决的。 使实验室能够揭示有关基因组完整性机制的新概念。 这种新颖的单分子方法的使用取决于获得高度纯化的 该补充设备请求将使我们能够更换我们的重组蛋白。 过时的蛋白质纯化系统，以便我们能够继续推进我们的研究 了解基因组完整性工作的机制。