ZellScanner ONE

泽尔扫描仪一号

• 批准号: 10176806
• 负责人: Lisa Borghesi
• 金额: $ 28.68万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176806
• 关键词: Architecture; Area; Biological Sciences; Biology; Blood Vessels; Caring; Cells; Direct Costs; Discipline; Ensure; Flow Cytometry; Funding; Hour; Human Resources; Image; Immune; Immune System Diseases; Immunology; Individual; Infrastructure; Institutes; Microscopy; Minor; Oncology; Phase; Positioning Attribute; Productivity; Research; Research Personnel; Rheumatology; Sister; System; Time; Tissues; Training; Transplantation; Universities; Work; cohort; experience; experimental study; imaging platform; insight; instrument; medical schools

Project Summary/Abstract This request is for funds to purchase a Canopy Biosciences ZellScanner One high-multiplexing chip cytometer. There is no ZellScanner imaging platform at the University of Pittsburgh or within 100 miles. Unique as an instrument, the ZellScanner system combines the strengths of multiparameter flow cytometry (marker co-expression, quantitative, sensitive) with microscopy (positional information, tissue architecture, subcellular detail) to permit analysis of highly defined immune subsets and their spatial positioning in the native tissue microenvironment. Such high content imaging allows for deep insights into immune cell subpopulations in the context of tissue localization. The instrument will be housed within the Unified Flow Cytometry Core, a centralized core, at the University of Pittsburgh School of Medicine. The Unified Flow Core currently serves over 180 Pis and 500 individual Users. For the proposed instrument, Major Users include 13 Pis (10 NiH funded) with another 5 Pis as Minor Users. Together, these Pis have over $20 M in NiH-funded direct costs from at least 4 different institutes. A major research focus is in the broad area of immunology and allied immune diseases, with sister disciplines such as transplantation, rheumatology, vascular biology, and oncology also well represented. While the Unified Flow Cytometry Core is well-endowed with traditional (though busy) analyzers and sorters, the ZellScanner chip cytometer brings totally new breadth to our research capabilities. Our preliminary and demo work proved to us that this instrument will truly allow our investigators to break new ground in their research. The demo phase of this instrument indeed created great excitement among the Pis and hands-on Users. The new machine will be well cared for by the staff of the Unified Flow Cytometry Core, which has an extensive infrastructure and culture in place to train Users, oversee the machine and ensure that it is self-supporting. Two experienced core personnel will be responsible for the conceptual and day to day aspects of experiments on the ZellScanner unit. We will start and certify the machine each morning and troubleshoot any problems, as we will do at any time during working hours. As such, it will have a remarkable impact on the productivity of an outstanding and well-funded cohort of PHS investigators.

项目摘要/摘要 该要求是要购买Canopy Biosciences Zellscanner的资金 细胞仪。匹兹堡大学没有Zellscanner成像平台或100次之内 英里。 Zellscanner系统独特地作为仪器，结合了 多参数流式细胞术（标记共表达，定量，灵敏） 显微镜（位置信息，组织结构，亚细胞细节）以允许分析 高度定义的免疫集及其在天然组织中的空间定位 微环境。如此高的内容成像可以深入了解免疫细胞 在组织定位的背景下的亚群。该仪器将安置在 匹兹堡大学的集中核心核心核心核心核心核心核心 医学。统一的流核目前为180多个PI和500个个人用户提供服务。为了 拟议的仪器，主要用户包括13个PI（10个NIH资助），另外5个PI作为次要用户。 这些PI共同拥有超过20 m的NIH资助直接费用至少4个 不同的机构。主要的研究重点是免疫学和联盟免疫的广泛领域 疾病，以及姊妹学科，例如移植，风湿病学，血管生物学， 肿瘤学也很好地代表了。而统一的流式细胞仪核心却充满了 传统（虽然很忙）分析仪和分类器，Zellscanner芯片细胞仪带来 我们的研究能力全新的广度。我们的初步和演示工作证明了 对我们来说，该工具将真正允许我们的调查人员在他们的 研究。该工具的演示阶段确实在PI和 动手用户。新机器将由统一流式细胞仪的工作人员很好地照顾 核心，具有广泛的基础设施和文化来培训用户，监督 机器并确保它是自支持的。两个经验丰富的核心人员将负责 对于ZellScanner单元上实验的概念和日常方面。我们将开始并 每天早晨证明机器并将任何问题进行故障，就像我们在 工作时间。因此，它将对 杰出且资金充足的PHS调查人员队列。