Special BD LSRFortessaTM

特别BD LSRFortessaTM

• 批准号: 8244704
• 负责人: Lisa Borghesi
• 金额: $ 38.35万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8244704
• 关键词: 7 year old; 8 year old; Area; Biomedical Research; Cell Separation; Cells; Color; Communities; Complex; Core Facility; Development; Flow Cytometry; Fortune; Funding; Goals; Housing; Human Resources; Immunologist; Immunology; Institution; Lasers; Leukocytes; Mediating; Medical; Palpable; Phenotype; Productivity; Request for Applications; Research; Research Personnel; Schedule; Supervision; Time; United States Environmental Protection Agency; United States National Institutes of Health; Universities; Viola; base; instrument; instrumentation; medical schools; medical specialties; operation; pathogen; research study; tool; tumor

DESCRIPTION (provided by applicant): This request is for funds to purchase a four laser BD LSRFortessa multiparameter flow cytometer. This instrument will be housed within the Department of Immunology Flow Cytometry Facility at the University of Pittsburgh School of Medicine. The goal of the core facility is to provide sensitive cell phenotyping and cell sorting capabilities to research groups within the University of Pittsburgh biomedical community. Multicolor flow cytometry is an essential tool that allows immunologists to dissect the development and coordinated interactions of the white blood cells that mediate pathogen defense and anti-tumor activity. The current facility contains an eight year old BD LSR II analytic instrument and a seven year old Aria cell sorter. Our sole analytic instrument is at saturated capacity for supervised applications. The main reason for this proposal is to provide critically needed instrumentation time, under expert supervision, for complex multi-color experiments. Since the Immunology Flow Cytometry Facility commenced operation eight years ago, it has become an integral part of the medical community with more than 40 PHS funded groups within the biomedical research area as well as PHS supported projects with investigators in other departments and at neighboring institutions. The sole multiparameter analytic flow cytometer we house is insufficient to accommodate the number of researchers. We have an immediate demand for a second analytic instrument that parallels our current four laser capabilities. We also have a growing need for a specialty laser, the yellow-green laser, for advanced applications. Therefore, this application requests a four laser BD LSRFortessa flow cytometer (blue, red, violet, UV) which will be supplemented by matching funds from the Department of Immunology for the purchase of a fifth laser (yellow-green). The proposed four laser LSRFortessa allows us to provide users with an instrument that 1) eases the scheduling overload of our analyzer, 2) enables facility personnel to help multiple users simultaneous thereby maximizing research productivity, and 3) with the addition of the yellow-green laser, will culminate in a five laser configuration that is not currently available at the University, and for which there is palpable demand. The PHS funded user base within the institution continues to expand, and Pitt is currently ranked 7th in NIH funding. The cytometer will be purchased from BD Biosciences, a company that ranks 10th among Fortune 500 companies that participate in the US Environmental Protection Agency's Green Power Partnership.

描述（由申请人提供）：此请求是要购买四个激光BD LSRFORTESSA多参数流细胞仪。该仪器将安置在匹兹堡大学医学院免疫学流式细胞仪设施中。核心设施的目的是为匹兹堡大学生物医学界的研究小组提供敏感的细胞表型和细胞分选功能。多色流式细胞仪是必不可少的工具，可让免疫学家剖析介导病原体防御和抗肿瘤活性的白细胞的发育和协调相互作用。当前的设施包含八岁的BD LSR II分析 仪器和一个七岁的咏叹调细胞分选仪。我们的唯一分析工具具有监督应用的饱和能力。该建议的主要原因是在专家监督下为复杂的多色实验提供急需的仪器时间。由于八年前的免疫学流式细胞仪设施开始运营，因此它已成为医学界不可或缺的一部分，其中有40多个PHS资助的群体，以及在其他部门和邻近机构的研究人员的PHS支持项目。我们所容纳的唯一多参数分析流式细胞仪不足以适应研究人员的数量。我们对第二种分析工具有直接的需求，该工具与当前的四个激光功能平行。对于高级应用，我们还越来越需要特种激光器，黄绿色激光器。因此，该应用程序要求使用四个激光BD LSRFTRESTESSA流式细胞仪（蓝色，红色，紫罗兰色，紫外线），该细胞仪将通过免疫学系的匹配资金来补充，以购买第五激光（黄绿色）。提出的四个激光LSRFortessa使我们能够为用户提供一种工具，以使我们的分析器的安排超负荷，2）使设施人员能够同时帮助多个用户，从而最大化研究生产力，3）随着黄色绿色激光的添加，将在五个Laser pal中加以限制。该机构内的PHS资助的用户群继续扩大，Pitt目前在NIH资金中排名第七。细胞仪将从BD Biosciences购买，该公司在参加美国环境保护局的绿色力量合作伙伴关系的财富500强公司中排名第十。

项目成果

Adenosine Triphosphate Released by Candida albicans Is Associated with Reduced Skin Infectivity.

白色念珠菌释放的三磷酸腺苷与皮肤感染性降低有关。

• DOI: 10.1016/j.jid.2021.03.010
• 发表时间: 2021
• 期刊: The Journal of investigative dermatology
• 作者: Zhang,Shiqun;Edwards,TaraN;Mogavero,Selene;Mathers,AliciaR;Hube,Bernhard;Berman,Judith;Bougnoux,Marie-Elisabeth;D'Enfert,Christophe;Kaplan,DanielH
• 通讯作者: Kaplan,DanielH

CD91 on dendritic cells governs immunosurveillance of nascent, emerging tumors.

树突状细胞上的 CD91 控制新生肿瘤的免疫监视。

• DOI: 10.1172/jci.insight.127239
• 发表时间: 2019
• 期刊: JCI insight
• 影响因子: 8
• 作者: Sedlacek,AbigailL;Younker,TheodoreP;Zhou,YuJerry;Borghesi,Lisa;Shcheglova,Tatiana;Mandoiu,IonI;Binder,RobertJ
• 通讯作者: Binder,RobertJ

In vivo induction of regulatory T cells promotes allergen tolerance and suppresses allergic contact dermatitis.

• DOI: 10.1016/j.jconrel.2017.07.006
• 发表时间: 2017-09-10
• 期刊: JOURNAL OF CONTROLLED RELEASE
• 影响因子: 10.8
• 作者: Balmert, Stephen C.;Donahue, Cara;Vu, John R.;Erdos, Geza;Falo, Louis D., Jr.;Little, Steven R.
• 通讯作者: Little, Steven R.