Cryo Transmission Electron Microscope for SPA, Cryo-ET and MicroED studies at UCSB

UCSB 用于 SPA、Cryo-ET 和 MicroED 研究的冷冻透射电子显微镜

• 批准号: 10177740
• 负责人: DORIT HANEIN
• 金额: $ 200万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177740
• 关键词: Appointment; Architecture; Area; Automobile Driving; Biochemistry; Biocompatible Materials; Biological; Biology; Biomedical Engineering; California; Characteristics; Chemistry; Communities; Cryoelectron Microscopy; Electron Microscope; Electron Microscopy Facility; Engineering; Faculty; Funding; Knowledge; Laboratories; Leadership; Life; Micro Electron Diffraction; Molecular; Molecular Chaperones; Nucleic Acids; Operations Research; Permeability; Process; Proteins; Research; Resolution; Running; Science; Structural Biochemistry; Structure; System; Talents; Therapeutic; United States National Institutes of Health; Universities; Visualization; base; biological systems; college; doctoral student; electron tomography; inhibitor/antagonist; insight; instrumentation; interdisciplinary collaboration; particle; programs; protein function; structural biology; transmission process

PROJECT SUMMARY/ABSTRACT With the purchase of a GlaciosTM transmission electron microscope system we seek to establish the first single particle analysis (SPA) cryo-electron microscopy (cryo-EM), -electron tomography (-ET) and micro-electron diffraction (micro- ED) facility at the University of California Santa Barbara, and the only one on the California Central Coast: the UCSB Structural Biology cryo-Electron Microscopy (SB2EM) facility. Dedicated biological cryo-EM capabilities will become the linchpin to UCSB’s strong leadership and strength in quantitative biology, structural biochemistry and bioengineering at the interdisciplinary interface. Nowhere is the boundary between Departments, Colleges and Programs more permeable than at UCSB: faculty advise PhD students of all Science and Engineering Departments with no to little barriers, dual appointments in more than one department is the norm at UCSB and research operations relying on high-end and state-of-the-art instrumentation in professionally run shared facilities is a forte of the UCSB campus. The SB2EM facility will be integrated into this culture of interdisciplinary collaboration, as well as a network of first-rate shared facilities and laboratories across our campus’s largely merged science and engineering community. The impact of SB2EM on the current NIH funded UCSB faculty will be direct, immediate and dramatic, but it will also go well beyond the currently funded NIH projects by inspiring and enabling entirely new areas of biomedically inspired research, and the hiring of new faculty talent. The ability to the “turn the lights on” to directly see the molecular machinery of life fundamentally changes how we understand and, ultimately, manipulate biological materials, systems and processes. Cryo-EM brings atomic- and molecular- level information, typically the core approach of a chemist, into biology and bioengineering, and vice versa, through, for example, the visualization of how molecular chaperones untangle neurofibrillary plaques, or through the structure-based engineering of molecular inhibitors and architectures built of protein and nucleic acid building blocks. The emergence of unprecedentedly high-resolution structural insights into biological molecules and systems offers a critical competitive advantage towards understanding their functions, discovering function-driving mechanisms, and engineering therapeutic solutions. The study of the protein function will be dramatically facilitated and the re-engineering of biological systems vastly enhanced with knowledge of the underlying structure and structural characteristics. It is this insight that led us to prioritize structure-based biochemistry, bioorganic chemistry, biology, and bioengineering at UCSB.

项目概要/摘要 通过购买 GlaciosTM 透射电子显微镜系统，我们寻求建立第一个单粒子 分析（SPA）冷冻电子显微镜（cryo-EM）、电子断层扫描（-ET）和微电子衍射（micro- 加州大学圣塔芭芭拉分校的 ED）设施，也是加州中央海岸唯一的设施：UCSB 结构生物学冷冻电子显微镜 (SB2EM) 设施将成为专用的生物冷冻电子显微镜功能。 UCSB 在定量生物学、结构生物化学和生物工程领域的强大领导力和实力的关键 院系、学院和项目之间的跨学科界面没有什么比这里更具有渗透性的了。 加州大学圣巴巴拉分校：教师为所有科学和工程系的博士生提供毫无障碍、双重任命的指导 多个部门在 UCSB 是一种常态，研究运作依赖于高端和最先进的技术 专业运营的共享设施中的仪器是 UCSB 校园的强项，SB2EM 设施将被整合。 融入这种跨学科合作的文化，以及一流的共享设施和实验室网络 我们校园的科学和工程社区很大程度上合并了 SB2EM 对当前 NIH 资助的 UCSB 的影响。 教师将是直接的、直接的和戏剧性的，但它也将远远超出目前资助的 NIH 项目，通过启发 并实现生物医学启发的全新领域的研究，以及聘用新的教师人才。 “打开灯”直接看到生命分子机制的能力从根本上改变了我们的方式 理解并最终操纵生物材料、系统和过程。 水平信息，通常是化学家的核心方法，进入生物学和生物工程，反之亦然，通过， 例如，分子伴侣如何解开神经原纤维斑块的可视化，或通过基于结构的 由蛋白质和核酸构件构建的分子抑制剂和结构的工程的出现。 对生物分子和系统的前所未有的高分辨率结构洞察提供了关键的竞争优势 对其功能的优势理解、发现功能驱动机制和工程治疗 蛋白质功能的研究和生物系统的重新设计将得到极大的促进。 正是这种洞察力使我们对底层结构和结构特征的了解大大增强。 UCSB 优先考虑基于结构的生物化学、生物有机化学、生物学和生物工程。