Instrumentation Upgrade: acquisition of an intermediate voltage TEM

仪器升级：获取中间电压 TEM

• 批准号: 8335214
• 负责人: DORIT HANEIN
• 金额: $ 188.5万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8335214
• 关键词: Area; Automation; Biological; Cells; Communicable Diseases; Data Quality; Development; Developmental Biology; Disease model; Electron Microscope; Equipment; Funding; Grant; Health; Health Sciences; Image; In Situ; Inflammation; Institutes; Medical Research; Microscope; Molecular; Natural Immunity; Neoplasm Metastasis; New Mexico; Output; Physiologic calcification; Research Institute; Research Personnel; Resolution; Sampling; Scientist; Screening procedure; Series; Staging; Structure; Temperature; United States National Institutes of Health; Universities; Virginia; data acquisition; electron optics; instrumentation; macromolecular assembly; medical schools; novel therapeutics; tomography; transmission process; voltage

DESCRIPTION (provided by applicant): PROJECT SUMMARY Here, we seek funding to purchase a modern, intermediate voltage (300 kV) transmission electron microscope (F30 Polara). This microscope will replace the existing FEI Tecnai F20 at Sanford-Burnham Medical Research Institute (SBMRI). The F30 will be integrated into an existing unit that comprises of cryo-TEMs and ancillary equipment. It will provide the means for a more robust, high-quality/high-throughput cryo-tomography data acquisition at LN2 temperatures by semi-automation of sample handling and more robust screening capabilities, better and more powerful electron optics, LN2 stable stage and higher rates of tilt series completion. Together, this upgrade will allow us to optimize data quality and resolution for a broad range of biological samples from our scientific teams, enabling us to correlate molecular mechanisms and functional outputs, to provide scale-integrated models of disease, and to provide new perspectives for the development of new therapeutics. The proposed instrumentation upgrade will support teams of scientists from SBMRI and researchers from TSRI, UCSD, UCLA, Yale University, University of Virginia School of Medicine, Harvard Medical School, Stowers Institute for Medical Research, and the University of New Mexico Health Sciences Center. All these researchers are funded through project grants from the NIH to pursue a broad range of health-related studies in the areas of developmental biology, inflammation, innate immunity, bone mineralization, metastasis, and infectious diseases. All of the projects highlighted in this application employ cryo-tomography imaging, ranging from tomography of single macromolecular assembly structure determination to in-situ whole cell tomography. These projects are only representatives of a potentially much larger pool of projects that could greatly benefit from access to this dedicated, modern cryo-tomography F30 scope.

描述（由申请人提供）： 项目摘要 在此，我们寻求资金购买一台现代化的中压 (300 kV) 透射电子显微镜 (F30 Polara)。该显微镜将取代桑福德伯纳姆医学研究所 (SBMRI) 现有的 FEI Tecnai F20。 F30 将集成到由冷冻 TEM 和辅助设备组成的现有装置中。它将通过半自动化样品处理和更强大的筛选功能、更好、更强大的电子光学、液氮稳定台和更高的性能，提供在液氮温度下进行更稳健、高质量/高通量的冷冻断层扫描数据采集的方法。倾斜系列完成率。总之，此次升级将使我们能够优化科学团队的各种生物样本的数据质量和分辨率，使我们能够将分子机制和功能输出关联起来，提供疾病的规模集成模型，并为研究提供新的视角。新疗法的开发。拟议的仪器升级将为 SBMRI 的科学家团队以及来自 TSRI、加州大学圣地亚哥分校、加州大学洛杉矶分校、耶鲁大学、弗吉尼亚大学医学院、哈佛医学院、斯托尔斯医学研究所和新墨西哥大学健康科学中心的研究人员团队提供支持。所有这些研究人员均通过美国国立卫生研究院 (NIH) 的项目拨款资助，在发育生物学、炎症、先天免疫、骨矿化、转移和传染病等领域开展广泛的健康相关研究。本申请中强调的所有项目均采用冷冻断层扫描成像，范围从单个大分子组装结构测定的断层扫描到原位全细胞断层扫描。这些项目只是潜在更大项目池的代表，这些项目可以从使用这款专用的现代冷冻断层扫描 F30 示波器中获益匪浅。

项目成果

Morphological control enables nanometer-scale dissection of cell-cell signaling complexes.

• DOI: 10.1038/s41467-022-35409-9
• 发表时间: 2022-12-20
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Dow, Liam P.;Gaietta, Guido;Kaufman, Yair;Swift, Mark F.;Lemos, Moara;Lane, Kerry;Hopcroft, Matthew;Bezault, Armel;Sauvanet, Cecile;Volkmann, Niels;Pruitt, Beth L.;Hanein, Dorit
• 通讯作者: Hanein, Dorit

Characterization of heterogeneity in nanodisc samples using Feret signatures.

使用 Feret 特征表征纳米圆盘样品的异质性。

• DOI: 10.1016/j.jsb.2022.107916
• 发表时间: 2022
• 期刊: Journal of structural biology
• 影响因子: 3
• 作者: Vilela,Fernando;Bezault,Armel;RodriguezdeFrancisco,Borja;Sauvanet,Cécile;Xu,Xiao-Ping;Swift,MarkF;Yao,Yong;Marrasi,FrancescaM;Hanein,Dorit;Volkmann,Niels
• 通讯作者: Volkmann,Niels