ICP-QQQ

ICP-MS/MS

• 批准号: 10177184
• 负责人: Ana Maria Rule
• 金额: $ 34.95万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177184
• 关键词: 14 year old; American Indians; Arsenic; Biomedical Research; Clinical Investigator; Communities; Coupled; Data; Digestion; Discipline; Electronic cigarette; Environmental Engineering technology; Environmental Health; Environmental Pollutants; Equipment; Evaluation; Exposure to; Funding; Glioblastoma; Goals; Grant; Health; Heart Diseases; High Pressure Liquid Chromatography; Immune response; Inductively Coupled Plasma Mass Spectrometry; Infection; Influenza; Magnetism; Malignant Neoplasms; Malignant neoplasm of liver; Medical; Medicine; Metal exposure; Metals; Minor; Pilot Projects; Population Heterogeneity; Prevention; Public Health; Public Health Schools; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Risk Factors; Services; Stroke; Students; System; Technical Expertise; Technology; Toxic effect; Trace metal; Translations; United States National Institutes of Health; Universities; cancer survival; equipment acquisition; experience; hyperthermia treatment; instrument; instrumentation; mass spectrometer; medical schools; microwave electromagnetic radiation; novel therapeutic intervention; particle; rapid growth; school health; tumor

Project Summary/Abstract: The overall goal of this proposal is to acquire funds to purchase a triple quadrupole mass spectrometer and a new microwave digestor to replace a 14-year old Agilent 7500ce ICP-MS and a MARS5 microwave. The funds will be used to purchase an Agilent 8900 ICP-QQQ system that can be coupled with our existing Agilent 1260 HPLC system which will be dedicated to support NIH-funded investigators requiring quantitative metal analysis, including metal speciation. A group of investigators (6 major, 5 minor) has been identified whose research (75.1% NIH-funded) will greatly benefit from analyses performed with the requested instrumentation. The Trace Metals Lab (TML) in the Department of Environmental Health and Engineering at Johns Hopkins Bloomberg School of Public Health houses the only ICP-MS in the medical campus, which includes the School of Medicine (SOM) and the School of Public Health. The TML has been providing accurate, sensitive, and reliable trace metal analysis for health-related research since 2006. This shared resource provides funded investigators with technical expertise and access to large equipment for quantitative analysis of trace metals. The JHU TML is used heavily by NIH-funded grants supporting research that ranges from exposure to metals from e-cigarettes (R01), evaluation of Magnetic Hyperthermia Therapy (MHT) against infiltrating glioblastoma (R01) and liver cancer tumors (R01), assessment of arsenic exposure on immune responses to influenza (R01), Arsenic and other metal exposures in American Indian communities (R01), among others. The research that will benefit from this instrumentation will have enormous impacts on the disciplines of Medicine and Public Health. With ICP- QQQ, HPLC, microwave digestion, and Single Particle analysis capabilities, our lab will continue to advance the translation of basic biomedical research to treatments and cures, and will also contribute to prevention through strategies for reducing exposure to environmental pollutants for diverse populations. The number of basic and clinical investigators requiring analytical services of trace metals has experienced a rapid growth. As a result, usage of the existing ICP-MS instrument is currently at full capacity. The new instrumentation will also benefit studies that are not currently NIH funded, pilot projects to collect data for NIH grants, and student research projects. The ICP-MS capability of the JHU TML will also contribute to the long-range biomedical research goals of the Johns Hopkins University. This instrumentation will benefit research to understand basic cellular toxicity, risk factors for infection, develop important new therapeutic strategies against cancer, and advance our understanding of stroke mechanisms, heart disease, cancer survival, and immune responses. As outlined in this application, acquisition of this equipment will make this technology available to a large number of investigators, continuing with the goals of the Trace Metals Lab, the Department of Environmental Health and Engineering, the Bloomberg School or Public Health, the Johns Hopkins University, and the Biomedical community as a whole.

项目摘要/摘要： 该提案的总体目标是获取资金以购买三倍四极杆质谱仪和 新的微波炉摘要，以取代14岁的Agilent 7500CE ICP-MS和MARS5微波炉。资金 将用于购买可以与我们现有的Agilent 1260结合的Agilent 8900 ICP-QQQ系统 HPLC系统将致力于支持NIH资助的研究人员，需要定量金属分析， 包括金属形成。已经确定了一组研究人员（6个专业，5个未成年人）的研究 （NIH资助的75.1％）将从请求的仪器进行的分析中受益匪浅。痕迹 约翰·霍普金斯（John Hopkins）彭博社环境健康与工程系的金属实验室（TML） 公共卫生学院拥有医学校园中唯一的ICP-MS，其中包括医学院 （SOM）和公共卫生学院。 TML一直提供准确，敏感和可靠的痕量金属 自2006年以来与健康相关研究的分析。该共享资源为资助的研究人员提供了 技术专长和使用大型设备，用于定量痕量金属。 jhu tml是 由NIH资助的赠款大量使用，支持研究范围从电子烟的金属接触不等 （R01），对浸润胶质母细胞瘤（R01）和肝脏的磁性高温治疗（MHT）的评估 癌症肿瘤（R01），对砷对流感的免疫反应（R01），砷和砷的评估 美洲印第安人社区（R01）等其他金属暴露。将受益的研究 从这种仪器中，将对医学和公共卫生的学科产生巨大影响。与ICP- QQQ，HPLC，微波消化和单个粒子分析功能，我们的实验室将继续推进 将基本生物医学研究转换为治疗和治疗方法，也将通过 减少对不同人群的环境污染物的暴露策略。基本和 需要痕量金属分析服务的临床研究人员经历了快速增长。因此， 现有的ICP-MS仪器的使用目前已满负荷。新仪器也将受益 目前尚未资助NIH的研究，试点项目以收集NIH赠款的数据以及学生研究 项目。 JHU TML的ICP-MS功能也将有助于远程生物医学研究目标 约翰霍普金斯大学。该仪器将有益于理解基本细胞毒性的研究， 感染的危险因素，制定针对癌症的重要新治疗策略，并促进我们的 了解中风机制，心脏病，癌症存活和免疫反应。如其中所述 应用，收购此设备将使这项技术可用于大量调查人员， 继续进行微量金属实验室的目标，环境健康与工程系， 彭博学校或公共卫生，约翰·霍普金斯大学以及整个生物医学界。

项目成果

Residential proximity to metal emitting industries and toenail metal concentration in the US Gulf States.

美国海湾国家的住宅靠近金属排放工业和趾甲金属集中区。

• DOI: 10.21203/rs.3.rs-3210942/v1
• 发表时间: 2023
• 期刊: Research square
• 作者: Lin,JoyceJy;Werder,Emily;Lawrence,KaitlynG;Jackson2nd,WBraxton;Sandler,DaleP;Dickerson,AishaS;Engel,LawrenceS;Rule,AnaM
• 通讯作者: Rule,AnaM