Action and interaction of ionotropic and metabotropic neurotransmission

离子型和代谢型神经传递的作用和相互作用

• 批准号: 10166240
• 负责人: Bernardo L Sabatini
• 金额: $ 2.35万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10166240
• 关键词: Acetylcholine; American; Bachelor' s Degree; Biological Assay; Biology; Biomedical Research; Brain; Cameroon; Cyclic AMP-Dependent Protein Kinases; Development; Development Plans; Doctor of Philosophy; Educational workshop; Enrollment; Ethnic group; Goals; Image; Measures; Mediator of activation protein; Mentors; Mentorship; Methods; Microglia; Morphology; NCI Scholars Program; Neuroglia; Neurons; Neurosciences; Neurosciences Research; Neurotransmitters; Postdoctoral Fellow; Preparation; Research; Schools; Signal Transduction; Signaling Molecule; Slice; Testing; Training; Underrepresented Groups; Universities; Woman; career; career development; design; falls; gamma-Aminobutyric Acid; laboratory experience; meetings; neurotransmission; parent grant; programs; receptor; research and development; response; symposium; tutoring

Abstract This is an application for an administrative diversity supplement to R37 NS046579-16, “Action and interaction of ionotropic and metabotropic neurotransmission” to support the scientific training and development of Rochinelle Dongmo. Ms. Dongmo is a baccalaureate degree holder from Emory University who is currently enrolled in a post-baccalaureate research scholars program at Harvard to prepare for Ph.D. graduate programs in neuroscience, with the goal of enrolling in a program by the Fall of 2021. As a black woman who is a U.S. permanent resident currently finishing her naturalization as an American citizen from Cameroon, she is part of an ethnic group underrepresented in biomedical research. Ms. Dongmo's research in the lab will pursue two aims related to the parent grant, both of them seeking to better understand the mechanism of metabotropic neurotransmission to non-neuronal microglia. In the first aim, Ms. Dongmo will develop an imaging assay to assess microglia activation in ex vivo brain slices via changes in gross morphology. This assay will allow us to test the activation of microglia to multiple neurotransmitters, most notably acetylcholine and GABA, for which microglia are known to possess metabotropic receptors. In the second aim, Ms. Dongmo will measure the effects of microglia activation on the intracellular activity of Ca2+ and protein kinase A (PKA), two central intracellular signaling molecules. She will test the Ca2+ and PKA activity in response to both known activators of microglia, as well as to GABA and acetylcholine, whose net effect on microglia activation will be determined in her first aim. She will be mentored during the execution of this research plan on both all scientific and technical aspects of the research. We have designed a comprehensive mentorship and training plan for Ms. Dongmo including lab experience, regular one-on-one meetings with the PI and a senior post-doc, conference presentations, all in addition to her participation in the Harvard Research Scholars Initiative, which includes classes, workshops, tutoring, GRE test preparation, and graduate school application guidance. These research and career development plans will prepare Ms. Dongmo for a successful academic career in research neuroscience.

抽象的 这是R37 NS046579-16的行政多样性补充的应用程序，“行动和互动 离子型和代谢性神经传递”，以支持Rochinelle的科学培训和发展 dongmo。 Dongmo女士是埃默里大学（Emory University）的学士学位持有人 哈佛大学后的学者研究学者计划为博士做准备。研究生课程 神经科学，目的是在2021年秋季参加一项计划。作为美国的黑人妇女 她目前从喀麦隆（Cameroon）成为美国公民的归化居民，她是 一个族裔群体在生物医学研究中的人数不足。 Dongmo女士在实验室的研究将追求与父母赠款有关的两个目标，他们俩都在寻求改善 了解非神经元小胶质细胞的代谢性神经传递的机制。在第一个目标中，女士 Dongmo将开发成像评估，以通过总体变化来评估离体脑切片中的小胶质细胞激活 形态学。该测定将使我们能够测试小胶质细胞对多个神经递质的激活，最著名的是 乙酰胆碱和GABA，已知小胶质细胞具有代谢型受体。在第二个目标中 Dongmo女士将测量小胶质细胞激活对Ca2+和蛋白激酶细胞内活性的影响 A（PKA），两个中央的细胞内信号分子。她将对CA2+和PKA活动进行响应 小胶质细胞的已知激活剂，以及GABA和乙酰胆碱，其净作用对小胶质细胞激活 将在她的第一个目标中确定。在执行本研究计划期间，她将被考虑 研究的科学和技术方面。我们设计了一种全面的心态和培训 Dongmo女士的计划，包括实验室经验，与PI的定期一对一会议和高级大约会议， 会议演讲，除了她参加哈佛研究学者倡议之外 包括课程，研讨会，辅导，GRE测试准备和研究生院申请指导。这些 研究和职业发展计划将为Dongmo女士做好准备，以成功地研究研究 神经科学。