Mechanisms of Visual Encoding and Plasticity in Anterior Cingulate Cortex

前扣带皮层视觉编码和可塑性机制

基本信息

批准号：
10161051
负责人：
Michael S Sidorov
金额：
$ 24.9万
依托单位：
CHILDREN'S RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-30 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10161051
关键词：
Angelman Syndrome Anterior Area Basic Science Behavior Behavioral Biological Models Boston Brain Calcium Chronic Clinical Contralateral Data Defect Detection Development Plans Disease Educational process of instructing Emotional Eye Faculty Foundations Genes Genetic Models Goals Grant Image Impairment Individual Ipsilateral Knowledge Lateral Geniculate Body Learning Longitudinal Studies Measures Mentors Modification Monitor Mus Neocortex Neurodevelopmental Disorder Neurons Neurosciences Research Oranges Pattern Phase Phenotype Positioning Attribute Prefrontal Cortex Process Property Research Research Personnel Secure Sensory Slice Stimulus Syndrome Testing Thalamic structure Training Translational Research UBE3A gene Universities Vision Visual Visual Cortex Vocational Guidance Work Writing area striata autism spectrum disorder behavioral impairment behavioral phenotyping career career development cingulate cortex cognitive control experience experimental study gain of function mutation improved in vivo loss of function mutation mouse model novel optogenetics programs response risk variant sequence learning skills tenure track tool two-photon visual information visual process visual processing visual stimulus

项目摘要

PROJECT SUMMARY/ABSTRACT Mouse primary visual cortex (V1) is capable of encoding visual stimuli and undergoing bidirectional plasticity coincident with patterned visual experience. The prefrontal anterior cingulate cortex (ACC) receives input from visual cortex, but mechanisms of visual encoding and experience-dependent plasticity in ACC are not well- understood. The goal of the proposed research is to define how ACC encodes and adapts to patterned visual experience. During the K99 phase, I will pair in vivo and ex vivo recordings with optogenetic and chemogenetic circuit manipulations to study how ACC microcircuits encode visual input. K99 training in two-photon calcium imaging will enable longitudinal studies of how individual neurons adapt to visual experience during the R00 phase. As abnormal sensory processing is a common feature of autism spectrum disorder, further R00 research will use approaches developed during K99 training to assess ACC microcircuit function in single-gene mouse models of autism. My long-term career goals are to advance basic knowledge of visual processing and to improve the lives of individuals with neurodevelopmental disorders through a collaborative program of basic and translational research. To achieve my goal of securing a tenure track faculty position as an independent researcher in neuroscience research, I have developed a comprehensive career development plan with the support of co-mentors Dr. Ben Philpot and Dr. Spencer Smith (UNC) and collaborators Dr. Paul Manis (UNC) and Dr. Jeff Gavornik (Boston University). This plan includes a) technical training in two-photon imaging, b) collaborative projects, c) training in presentation skills, d) training in mentoring skills, e) training in grant writing, f) career guidance, g) clinical exposure, h) formal coursework, i) teaching opportunties, and j) progress monitoring and evalulation.

项目摘要/摘要小鼠初级视觉皮层（V1）能够编码视觉刺激并进行双向可塑性与图案的视觉体验一致。前额叶前扣带回皮层（ACC）从视觉皮层，但是ACC中视觉编码和经验依赖性可塑性的机制不是很好理解。拟议的研究的目的是定义ACC编码和适应图案视觉的如何编码经验。在K99阶段，我将体内和离体记录与光遗传学和化学遗传学配对电路操作研究ACC微电路如何编码视觉输入。 K99在两光子钙中训练成像将使对单个神经元在R00期间如何适应视觉体验的纵向研究阶段。由于异常感觉处理是自闭症谱系障碍的常见特征，因此进一步r00 研究将使用在K99培训期间开发的方法来评估单基因中的ACC微电路功能自闭症的鼠标模型。我的长期职业目标是提高视觉处理的基本知识和通过基本的协作计划改善神经发育障碍的人的生活和翻译研究。为了实现我的目标，即确保作为独立的任期教师职位神经科学研究的研究人员，我已经制定了一项全面的职业发展计划联合会家Ben Philpot博士和Spencer Smith博士（UNC）和合作者Paul Manis（UNC）的支持和杰夫·加沃尼克（Jeff Gavornik）博士（波士顿大学）。该计划包括a）两光子成像中的技术培训，b）协作项目，c）演示技巧培训，d）指导技能的培训，e）授予写作的培训， f）职业指导，g）临床暴露，h）正式课程，i）教学机会和j）进步监视和评估。