Administrative Supplement To Incorporating the Microbiome into DR2 Activities to Inform Health Outcomes

将微生物组纳入 DR2 活动以告知健康结果的行政补充

• 批准号: 10162223
• 负责人: Joseph Frank Petrosino
• 金额: $ 40万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-07 至 2021-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10162223
• 关键词: Administrative Supplement; Affect; Air; Allergic; Biological; Biological Markers; Chemical Exposure; Chemicals; Cohort Studies; Collaborations; Collection; Communities; Data; Decision Making; Development; Disasters; Effectiveness; Environment; Environmental Exposure; Environmental Monitoring; Feces; Floods; Future; Genes; Health; Home environment; Human; Hurricane; Immunity; Individual; Link; Long-Term Effects; Metagenomics; Molds; Nose; Oral; Outcome; Outcome Assessment; Parents; Participant; Petroleum; Play; Questionnaires; Research; Research Design; Resources; Respiratory System; Role; Sampling; Symptoms; Testing; Time; Virus; Water; Whole-Genome Shotgun Sequencing; Work; bacteriome; base; cohort; experience; fungus; gut microbiome; gut microbiota; health assessment; improved; metagenomic sequencing; microbiome; microbiome analysis; microbiome research; monitoring device; mycobiome; novel; pathogen; remediation; response; superfund site; trend

PROJECT ABSTRACT The record flooding that occurred in Houston after Hurricane Harvey caused uncontrolled releases into the environment from over a dozen Superfund sites and several chemical/petroleum facilities. Under our Parent Time-sensitive R21, we worked with affected communities to administer health questionnaires, deployed wristbands to detect chemical exposures, and collected biosamples for microbiome analysis at 1- and 12-months after flooding. In these initial studies we identified a novel microbiome:environment interaction linked to allergic health outcomes. Under this Administrative Supplement, we will build on this work with longitudinal time- sensitive sampling of nasal, oral and gut biospecimens three years post-Hurricane Harvey. These biospecimens and microbiome data will be used for continuing disaster research response (DR2) activities, and stored as a unique resource for future microbiome:environment interaction studies. They will also be integrated with chemical exposure data obtained from wristband monitoring devices, and health outcome data from questionnaires administered to study participants at corresponding timepoints. Our Overarching Hypothesis is that microbiome:environment interactions play a role in the long-term health effects following a flood-related disaster. If correct, this would further support that the microbiome can serve as a target for such environmental exposures, or an indicator for actionable, post-disaster activities. In Specific Aim 1, we will collect environmental and biological samples from participants at the 3-year timepoint post-flooding, expanding our biospecimen collection to include samples reflective of a longer-term health outcomes. In Specific Aim 2, we will profile these samples via metagenomic sequencing and test whether microbiome-exposure or microbiome-health outcome relationships identified at earlier timepoints persist long-term. These include continued associations of the nasal mycobiome with fungi found in the home and the relationship between the gut microbial community, mold exposure, and allergic symptoms. Finally, in Specific Aim 3 we will integrate 1-month, 12-month and 3-year microbiome data with short- intermediate- and long-term health assessments and chemical exposure studies being conducted by our collaborators to identify novel microbiome:environment interactions. We will ask whether microbiome analyses from biospecimens collected over short-, medium-, and long-term correlate with chemical exposures captured by wristbands or other environmental monitoring and jointly inform health outcomes. In the future, these studies are expected to provide Preliminary Data for research aimed at 1) better understanding the role of the microbiome on post-disaster health outcomes, 2) identifying microbiome-based biomarkers of exposure(s) and/or health effects, and 3) improving decision- making by stakeholders and DR2 activities in response to future flood-related disasters.

项目摘要 哈维飓风造成了不受控制后，休斯敦发生的记录洪水 从十几个超级基金站点和几种化学/石油释放到环境中 设施。在我们的父母时间敏感R21下，我们与受影响社区合作 管理健康问卷，部署的腕带以检测化学暴露，以及 洪水后1个月和12个月收集生物样本进行微生物组分析。在这些最初 研究我们确定了一种新型微生物组：与过敏健康相关的环境相互作用 结果。根据这种行政补充，我们将以纵向时间为基础 Harvey后三年对鼻，口腔和肠道生物测量的敏感抽样。这些 生物测量和微生物组数据将用于持续灾难研究反应（DR2） 活动，并作为未来微生物组的独特资源存储：环境相互作用研究。 它们还将与从腕带监测获得的化学暴露数据集成 设备以及来自调查表的健康结果数据，用于研究参与者 相应的时间点。我们的总体假设是微生物组：环境 与洪水相关的灾难后，互动在长期健康影响中起作用。如果正确， 这将进一步支持微生物组可以作为这种环境的目标 暴露或可操作的污点后活动的指标。在特定目标1中，我们将收集 室内3年时间点的参与者的环境和生物样品， 扩展我们的生物循环收集以包括反映长期健康的样品 结果。在特定的目标2中，我们将通过宏基因组测序和测试来介绍这些样品 无论是微生物组暴露还是微生物组健康结果关系 时间表长期持续。其中包括鼻霉菌组的持续关联 真菌在家庭中发现以及肠道微生物社区之间的关系，霉菌 暴露和过敏症状。最后，在特定目标3中，我们将整合1个月，12个月 以及三年的微生物组数据，具有短期和长期健康评估， 我们的合作者正在进行化学暴露研究以识别新颖 微生物组：环境相互作用。我们将询问微生物组是否从 在短，中和长期与化学暴露相关的生物测量 由腕带或其他环境监测捕获，并共同告知健康结果。在 未来，这些研究有望为针对1）更好的研究提供初步数据 了解微生物组在污染后健康结果中的作用，2）识别 基于微生物组的暴露生物标志物和/或健康影响，3）改善决策 - 利益相关者和DR2活动响应未来与洪水有关的灾难。