HLA-A31-restricted gastric tumor antigenic peptide and clinical application

HLA-A31限制性胃肿瘤抗原肽及其临床应用

• 批准号: 09557019
• 负责人: SATO Noriyuki
• 金额: $ 6.02万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557019/
• 关键词: CTL; gastric tumor; HLA-A31; peptide; cancer vaccine; 抗原ペプチド; 癌ワクチン

1 HLA-A31-restricted antigenic peptide F4.2 (YSWMDISCWI) was identified by using human gastric tumor line HST-2 and autologous cytotoxic T lymphocyte (CTL) clone TcHST-2.2 F4.2 peptide could induce in vitro peptide-specific cytotoxic T lymphocytes (CTL) in approximately one third of HLA-A31 (+) gastric cancer patients.3 We cloned a candidate of cDNA C98 that encodes F4.2 peptide parental protein. C98 is a new gene, and is composed of 537 nucleotides with the open reading frame. However, C98 encodes YSWMDIITIC rather than YSWMDISCWI in its sequence. We are now underway to investigate how YSWMDIITIC was generated in the cells.

1 利用人胃肿瘤系 HST-2 和自体细胞毒性 T 淋巴细胞 (CTL) 克隆 TcHST-2.2 鉴定 HLA-A31 限制性抗原肽 F4.2 (YSWMDISCWI)，F4.2 肽可在体外诱导肽特异性细胞毒性 T大约三分之一的 HLA-A31 (+) 胃癌患者体内存在淋巴细胞 (CTL)。3 我们克隆了 cDNA C98 的候选者，它编码F4.2肽亲本蛋白。 C98是一个新基因，由537个核苷酸组成，具有开放阅读框。然而，C98 在其序列中编码 YSWMDIITIC 而不是 YSWMDISCWI。我们现在正在研究 YSWMDIITIC 是如何在细胞中产生的。

项目成果

佐原弘益,佐藤昇志,高橋延昭,菊地浩吉: "Annual Review免疫1998.中外医学社" 癌拒絶抗原ペプチドによる治療, 10 (1998)

Hiromasu Sahara、Noboru Sato、Nobuaki Takahashi、Hiroyoshi Kikuchi：“免疫学年度评论 1998。中外医学社”癌症排斥抗原肽治疗，10 (1998)

Kikuchi, K., and Sato, N.: "Cell-mediated Immunity to autologous cancer. Rationale of immunotherapy of human cancer with CTL.Gann Monogr.Japa.J.Cancer Res., In "Recent Advances of Human Tumor Immunology and Immunotherapy"" Gann Monogr.Jap.J.Cancer Res., E

Kikuchi, K. 和 Sato, N.：“细胞介导的自体癌症免疫。CTL 人类癌症免疫治疗的基本原理。Gann Monogr.Japa.J.Cancer Res.，在“人类肿瘤免疫学和免疫治疗的最新进展”中

Kamiguchi,K.,Sato,N.,Kikuchi,K.et al: "73 kD heat shock cognate protein is associated with transporter associated with antigen processing." Int.Immunol.(in press).

Kamiguchi,K.、Sato,N.、Kikuchi,K.et al：“73 kD 热休克同源蛋白与抗原加工相关的转运蛋白有关。”

Sato, N.and Torigoe, T.: "The molecular chaperones in cell cycle control. Stress of life - from molecules to man." Annals of New York Acad.Sci.851. 61-66 (1998)

Sato, N. 和 Torigoe, T.：“细胞周期控制中的分子伴侣。生命的压力 - 从分子到人。”

Sato N.and Torigoe,T.: "The molecular chaperones in the cell cycle control. In : Stress of life from molecules to man.ed.: P.Csermely" Annals of New York Academy of Sciences. 851. 61-66 (1998)

Sato N. 和 Torigoe,T.：“细胞周期控制中的分子伴侣。In：从分子到人的生命压力：P.Csermely”纽约科学院年鉴。