Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer

通过生物中心切换建立广谱耐药策略

基本信息

批准号：
09556071
负责人：
NAMBA Shigetou
金额：
$ 9.6万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09556071/
关键词：
high resistance broad spectrum resistance infectious transcript different virus species broad spectrum transgenic strategy 広域スペクトラムな抵抗性インフェクシャストランスクリプ不活性ミュータント polymerase cell-to-cell movement protein RNase serine-type protease P

项目摘要

For improving the research, we performed until now, by malting use of plant virus gene, mechanisms of resistance of virus infection were introduced and technique for detection was built. Within plant viruses known, more than 90% are RNA viruses. For gaining high resistance or broad spectrum resistance to these viruses, we performed our research and established related techniques. In this research project, based on our results attained these two years, gene function of Citrus tatter leaf capillovirus and several potyviruses were analyzed. Infectious transcripts were generated by using viral genes or non-coding regions of viral genomes with some modifications. The pathogenicity was analyzed when these infectious transcripts were inoculated to plants. We also constructed plant transformation vector. Several plant virus genes were engeneered into expression vector and then transformed into Ti plasmid. By transformingplant tissues by these constracts, transgenic plants were generated after differentiating and culturing the transformed plant cell. Each trangenic plant line was used for inoculation test with the same virus species or different species. And the level of resistance of each pair of plant line and inoculated virus was analyzed in detail. As a result, several interesting resistance lines were found. To improve broad spectrum resistance of transgenic strategy, more research must be done and might be profound.

为了改善研究，我们直到现在通过麦芽使用植物病毒基因，引入了病毒感染的抗性机制，并建立了检测技术。在已知的植物病毒中，超过90％是RNA病毒。为了获得对这些病毒的高耐药性或广泛的耐药性，我们进行了研究并建立了相关技术。在这项研究项目中，根据我们的结果，分析了柑橘破烂的叶囊病毒的基因功能和几个托管病毒。通过使用病毒基因组的病毒基因或具有一些修饰的病毒基因组的非编码区域产生传染性转录本。当将这些感染性转录本接种到植物时，分析了致病性。我们还构建了植物转化载体。几种植物病毒基因被连接到表达载体中，然后转化为Ti质粒。通过这些contracts转化组织，在区分和培养转化的植物细胞后产生了转基因植物。每个跨植物线系都用于与同一病毒或不同种类的接种测试。并详细分析了每对植物系和接种病毒的抗性水平。结果，发现了几条有趣的电阻线。为了提高转基因策略的广泛抗性，必须进行更多的研究，并且可能是深刻的。