Gene expression and control mechanisms of the plant mycoplasmas

植物支原体的基因表达及调控机制

• 批准号: 09460155
• 负责人: NAMBA Shigetou
• 金额: $ 8.83万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09460155/
• 关键词: plant mycoplasma; pathovar; Phytoplasma asteri; pathogen; microorganisms; insect vectors; bacteria; plant; 遺伝子制御ネットワーク; 野性株; シャトルベクター; 変異株; 分子分類; マイコプラズマ; パルスフィールド電気泳動; ローリングサークル型プラスミド; 一本鎖DNA結合タンパク質; 16S rRNA遺伝子; PCR増幅; 分子系統学的解析; 染色体D

Plant mycoplasmas (phytoplasmas) are plant pathogenic bacteria inhabiting in the plant cell cytoplasm and transmitted by insect vectors. Among all known microorganisms, they are the smallest in cell size and in genome size. This study was performed to elucidate molecular mechanisms of host determination of plant mycoplasmas and to established a system for gene introduction into the bacteria. The results are expected to contribute to the control of this pathogen which causes serious damage to more than 600 plant species.

植物支原体（植物植物）是居住在植物细胞质中的植物致病细菌，并由昆虫载体传播。在所有已知的微生物中，它们的细胞大小和基因组大小最小。进行这项研究是为了阐明植物支原体的宿主测定的分子机制，并建立了一种基因引入细菌的系统。预计该结果将导致对这种病原体的控制，从而对600多种植物物种造成严重损害。

项目成果

Kanazawa, T., Sawayanagi, T., Tanaka, M., Nishigawa, H., Kuboyama, T., Arai, S., Oshima, K., Matsuda, I., Tsuchizaki, T.: "Characterization of an isolated symptomatic onion yellows phytoplasma mutant."Japanese Journal of Mycoplasmology. 25. 121-123 (1998)

金泽，T.，泽柳，T.，田中，M.，西川，H.，久保山，T.，荒井，S.，大岛，K.，松田，I.，土崎，T.：“孤立的特征

Nishimura, Norio: "Transmission of cryptotaenia japonica witches' broom phytoplasma and oinion yellows phytoplasma by Hishimonus sellatus Uhler"Ann. Phytopath. Soc. Japan. 64. 474-477 (1998)

Nishimura，Norio：“由 Hishimonus sellatus Uhler 传播的隐带虫扫帚植原体和洋葱黄植原体”Ann。

Nishigawa,H.,Miyata,S.,Oshima,K.,Sawayanagi,T.,Komoto,A.,Kuboyama,T.,Matsuda,I.,Tsuchizaki,T.and Namba,S: "In planta expression of a protein encoded by the extrachromosomal DNA of phytoplasma and related to geminivirus replication proteins."Microbiology.

西川，H.，宫田，S.，大岛，K.，泽柳，T.，小本，A.，久保山，T.，松田，I.，土崎，T.和难波，S：“在植物中表达

Lu,X.,Hirata,H.,Ugaki,M.S.and Namba,S.: "Random mutagenesis in a plant viral genome using a DNA repair-deficient mutator Escherichia coli strain."Journal of Virological Methods. (in press). (2001)

Lu,X.、Hirata,H.、Ugaki,M.S. 和 Namba,S.：“使用 DNA 修复缺陷突变体大肠杆菌菌株对植物病毒基因组进行随机诱变。”病毒学方法杂志。

Komoto, A., Oshima, K., Kanazawa, T., Sawayanagi, T., Arai, S., Matsuda, I., Tsuchizai, T., Kuboyama, T.and Namba, S.: "Characterization of thymidylate kinase gene from OY-Phytoplasma."Japanese Journal of Mycoplasmology. 26. 37-39 (1999)

Komoto, A.、Oshima, K.、Kanazawa, T.、Sawayanagi, T.、Arai, S.、Matsuda, I.、Tsuchizai, T.、Kuboyama, T. 和 Namba, S.：“胸苷酸激酶的表征