Analysis on the signaling network involved in the host-switching mechanism of phytoplasmas

植原体宿主转换机制涉及的信号网络分析

• 批准号: 21248004
• 负责人: NAMBA Shigetou
• 金额: $ 27.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21248004/
• 关键词: 感染・増殖; ファイトプラズマ; 宿主因子; 感染機構

We investigated the molecular mechanism of phytoplasmal host switching to establish the basis of developing techniques to control the phytoplasma infection. Comparative analyses along with promoter analyses using phytoplasma isolate OY-M and its non-insect-transmissible mutant OY-NIM revealed that ORF3 located in the plasmid of phytoplasmas are involved in insect transmissibility. We developed phytoplasma DNA chip for the first time and performed microarray analysis to study host-specific regulation of phytoplasmal gene expressions. This analysis showed that phytoplasmas adapt to two diverse hosts, plants and insects, by dramatical alterations of their gene expression in response to the host.

我们研究了植物性宿主转换的分子机制，以建立开发控制植物性感染技术的基础。比较分析以及使用植物植物分离的OY-M和其非侵入式转移的突变体OY-NIM进行的启动子分析表明，位于植物质体质粒中的ORF3参与了昆虫的可传播性。我们首次开发了植物性DNA芯片，并进行了微阵列分析，以研究宿主特定的植物质基因表达调节。该分析表明，通过响应宿主的巨大改变，其基因表达的巨大改变，适应了两个不同的宿主，植物和昆虫。

项目成果

Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome

• DOI: 10.1016/j.virol.2009.10.008
• 发表时间: 2010-01-05
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Himeno, Misako;Maejima, Kensaku;Namba, Shigetou
• 通讯作者: Namba, Shigetou

Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana.

• DOI: 10.1186/1471-2229-12-103
• 发表时间: 2012-07-08
• 期刊: BMC plant biology
• 影响因子: 5.3
• 作者: Hashimoto M;Komatsu K;Maejima K;Okano Y;Shiraishi T;Ishikawa K;Takinami Y;Yamaji Y;Namba S
• 通讯作者: Namba S

Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection

• DOI: 10.1007/s00705-009-0571-x
• 发表时间: 2010-02
• 期刊: Archives of Virology
• 影响因子: 2.7
• 作者: Y. Yamaji;K. Sakurai;K. Hamada;K. Komatsu;J. Ozeki;A. Yoshida;A. Yoshii;T. Shimizu;S. Namba;T. Hibi

First report of Choanephora rot of ice plant (Mesembryanthemum crystallinum) caused by Choanephora cucurbitarum in Japan

• DOI: 10.1007/s10327-010-0249-y
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF GENERAL PLANT PATHOLOGY
• 影响因子: 1.2
• 作者: Kagiwada, Satoshi;Kayano, Yusuke;Namba, Shigetou
• 通讯作者: Namba, Shigetou

ファイトプラズマの主要抗原膜タンパク質遺伝子に認められる多様性

植原体主要抗原膜蛋白基因的多样性

• 发表时间: 2009
• 作者: 柿澤茂行;川西剛史;石井佳子;星朱香;石井良裕;小島信子;菅原杏子;大島研郎;難波成任
• 通讯作者: 難波成任