Defense mechanism for age-related macular degeneration.

年龄相关性黄斑变性的防御机制。

基本信息

批准号：
09671806
负责人：
OHIRA Akihiro
金额：
$ 1.98万
依托单位：
Shimane Medical University (1998)Nagasaki University (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671806/
关键词：
LIGHT DAMAGE REACTIVE OXYGEN INTERMEDIATES RETINA DEFENSE MECHANISM SUPEROXIDE DISMUTASE ANTIOXIDANTS ADULT T CELL LEUKEMIA DERIVED FACTOR GLUTATHIONE PEROXIDASE 抗酸化酵素

项目摘要

Manganese superoxide dismutase (Mn SOD) is a naturally occurring scavenger of superoxide, one of reactive oxygen intermediates. To determine if Mn SOD expression is enhanced as a defensive mechanism against oxidative challenges, such as intense light exposure, rats were exposed to cyclic tight (80 lux) for 2 weeks, intense light (1,800 lux) for 24 hours, and then again to cyclic light, Experimental and control (exposed to cyclic light only) eyes were enucleated 3 hours, 1, 3, 7, and 14 days after light challenge and protein expression was examined immunohistochemically using rabbit antisera against rat Mn SOD.There was no significant difference between the light-exposed and the control groups in the thickness of the outer nuclear layers. Both retinal pigment epithelial (RPE) cells and photoreceptor inner segments in the normal retina were labeled for Mn SOD.After Mn SOD labeling was lost 3 hours and day 1 after light challenge, it was re-expressed in the RPE cells days 3, 7, and 14 and in the photoreceptor inner segments the day 14 after light challenge in experimental animals. These results suggest that the retina might have a protective potential against light damage, in which Mn-SOD may play some important role.

锰超氧化物歧化酶（MN SOD）是一种天然存在的氧化剂，是反应性氧中间体之一。 To determine if Mn SOD expression is enhanced as a defensive mechanism against oxidative challenges, such as intense light exposure, rats were exposed to cyclic tight (80 lux) for 2 weeks, intense light (1,800 lux) for 24 hours, and then again to cyclic light, Experimental and control (exposed to cyclic light only) eyes were enucleated 3 hours, 1, 3, 7, and 14 days after light challenge and protein expression was examined使用兔抗血清对大鼠MN SOD进行免疫组织化学，在外核层厚度中，光暴露与对照组之间没有显着差异。 Both retinal pigment epithelial (RPE) cells and photoreceptor inner segments in the normal retina were labeled for Mn SOD.After Mn SOD labeling was lost 3 hours and day 1 after light challenge, it was re-expressed in the RPE cells days 3, 7, and 14 and in the photoreceptor inner segments the day 14 after light challenge in experimental animals.这些结果表明，视网膜可能具有防止轻损伤的保护潜力，其中MN-SOD可能会发挥重要作用。