Study on Molecular Mechanism and Therapy of Light-induce Retinal Damage

光致视网膜损伤的分子机制及治疗研究

基本信息

批准号：
14571673
负责人：
OHIRA Akihiro
金额：
$ 2.18万
依托单位：
Shimane University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571673/
关键词：
GPX 8OHdG TEM Light damage Retinal Pigment Epithelial Cells Photoreceptor PCR Apoptosis 80HdG グルタチオンペルオキシダーゼ抗酸化酵素

项目摘要

PURPOSE. To examine our hypothesis that glutathione peroxidase (GPX) is induced at different time points after retinal light exposure and localizes in different retinal cells.METHODS. The rats were kept cyclic light for 2 weeks before the experiments. The animals were maintained in 12-hour cycles of light and dark before and after exposure to intense white fluorescent light for as long as 24 hours and then returned to cyclic light. GPX expression was measured by immunohisto-cytochemistry, Western, and Northern blots. Light-induced retinal damage was determined by the outer nuclear layer (ONL) thickness relative to the total retinal thickness.RESULTS. GPX labeling was not seen in the photoreceptor inner segments and slight labeling was observed in the photoreceptor outer segments or the retinal pigment epithelial (RPE) cells in the normal retina kept in cyclic light. In retinal specimens maintained in light for 12 and 24 hours GPX labeling was induced in the photoreceptor outer segments … More and RPE cells. High GPX expression in the retinal pigment, epithelium was sustained until day 7 after challenge. In contrast, GPX expression in the photoreceptor outer segments decreased on day 1 and disappeared on days 3 and 7 after exposure. Intense GPX labeling was seen from the internal limiting membrane to fan lion cell layer. GPX labeling was constantly localized in both high-intensity white light and cyclic conditions, suggesting no induction of GPX in those areas. In addition, GPX labeling was apparent at the posterior retinal pole but not at the peripheral retina. We observe marked upregulation of GPX mRNA in rats kept in high-intensity white light. One, 3 and 7 days after exposure to high-intensity white light, there was a significant difference (P<0.0001) between the control and experimental groups in the ratio of the outer nuclear layer thickness to the entire retina.CONCLUSIONS. GPX was Induced at different time points after exposure to high-intensity white light and localized in different retinal cells. Changes in GPX expression after light exposure may, be related to the difference in light-damage susceptibility of the retina. Less

目的，我们的假设是，在光线曝光和视网膜细胞中的谷胱甘肽过氧化物酶（GPX）是在不同的时间点诱导的。在暴露于24小时的白色荧光之前和之后，光线和黑暗的循环是通过免疫疗法 - 偶像化学的表达来测量的。 SSSS t在某些NER段中的某些季节中，在光感受器的外部段或视网膜色素上皮（RPE）细胞中观察到一些较小的标记，在光视网膜上保持12和24小时的GPX标记中，在光感受器外部诱导了12和24小时的GPX标记。 TS…更多的RPE细胞在视网膜色素中，上皮在挑战后的第7天，GPX在第1天的光感受器外段中表达。从内部的膜到扇形细胞层。光线在高强度的白光之后，在不同的视网膜细胞中定位于HT暴露。