The development of new retinal oxidative stress market and the effects of vitamin E intensive care against retinal light damage

视网膜氧化应激新市场的发展及维生素E重症监护对抗视网膜光损伤的作用

• 批准号: 18591921
• 负责人: OHIRA Akihiro
• 金额: $ 2.46万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591921/
• 关键词: hydroxyoctadecadienoic acid(HODE); 8-iso-prostaglandin F2α; α-Tocopherol; α-Tocotrienol; α-Tocopherol transfer protein (α-TTP) (6); hydroxyoctadecadienoic acid(HODE); hydroxyoctadecadienoic acid

Maternal animals and pups of α-Tocopherol transfer protein (α-TTP) null mice were fed a vitamin E (VE) deficient diet for 4- or 18-months [VE (-) group]. Wild type. C57BL/6 mice were fed with 0.002 % α-tocopherol-supplemented diet [VE (+) group]. In retinas from 4-month-old VE (-) animals, the level of α-tocopherol, as measured by high performance liquid chromatography, was 71-fold lower than VE (+) group and γ-tocopherol was not detected, thus VE (-) group yielded more severe VE deficiency than ever reported. By gas chromatography analysis, n-3 pulyunsaturated fatty acids decreased (0.3- to 0.9-fold) in VE (-) mice, whereas other classes of fatty acids were unchanged or increased. When 18-month-old animals were compared to 4-month-old animals, levels of hydroxyoctadecadienoic acid and 8-iso-prostaglandin F^<2α>, biomarkers for lipid peroxidation, assessed by gas chromatography-mass spectrometry (GC-MS) were increased in retina and retinal pigment epithelium (RPE) from both VE (-) and (+) groups, whereas these age-related increases were more remarkable in VE (-) groups (2.6- to 43.5-fold) than VE (+) groups (0.8- to 8.7-fold). At 18-months of age, by light microscopy, number of outer nuclear layer (ONL) nuclei was 17 % lower in VE (-) than (+) groups (p<0.05). By electron microscopy, larger amounts of fibrous tissues between ONL nuclei, greatly expanded rod outer segment disks, and larger numbers of inclusion bodies in RPE (p<0.05) were observed in VE (-) group. By fluorescence microscopy, autofluorescence was increased in the RPE layer of VE (-) group. Severe deficiency of VE enhances increased lipid peroxidation and results in structural changes during aging in retina, indicating anti-aging roles of vitamin E.

α-生育酚转移蛋白（α-TTP）的幼体和幼体喂食维生素野生型4个月和VE（ - ）动物，高性能液相色谱的α-生育酚ED水平，未检测到比VE（+）组低71倍，并且未检测到生育酚，因此VE（ - ）组产生了更严重的VE缺乏报告。对于来自VE（ - ）和（+）组的脂质过氧化UM（RPE），与VE（+）组相比，VE（2.6至43.5倍）中与年龄相关的增长更为明显（0.8至8.7倍）。在VE（ - ）严重的RPE层中，荧光在VE（ - ）中增加了RPE（P <0.05）的大量扩展的杆段（P <0.05）。在视网膜期间的结构变化中，表明维生素E的抗衰老作用。

项目成果

Acceleration of age-related changes in retinal structure and biochemistry in vitamin E deficient diet-fed α-tocopherol transfer protein null mice

维生素 E 缺乏、α-生育酚转移蛋白无效小鼠的视网膜结构和生物化学与年龄相关的变化加速

• 发表时间: 2006
• 作者: Masaki;Tanito;Yasukazu;Yoshida;Sachiko;Kaidzu;Etsuo;Niki;Akihiro;Ohira
• 通讯作者: Ohira

Acceleration of Age-Related Changes in the Retina in α-tocopherol Transfer Protein Null Mice Fed a Vitamin E-Deficient Diet.

喂食缺乏维生素 E 的饮食的 α-生育酚转移蛋白无效小鼠的视网膜与年龄相关的变化加速。

• 发表时间: 2007
• 期刊: Invest Ophthalmol Vis Sci 48(1)
• 作者: Masaki;Tanito;Yasukadu;Yoshida;Sachiko;Kaidzu;Zhi-Hua;Chen;Osamu;Cynshi;Kou-ichi;Jishage;Etsuo;Niki;Akihiro;Ohira;Msdski Tatnito
• 通讯作者: Msdski Tatnito

Acceleration of Age-Related Changes in the Retina in α-Tocopherol Transfer Protein Null Mice Fed a Vitamin E-Deficient Diet

喂食缺乏维生素 E 的饮食的 α-生育酚转移蛋白无效小鼠视网膜与年龄相关的变化加速

• 发表时间: 2007
• 期刊: Investigative Ophthalmology & Vision Sicience 48(1)
• 作者: Uji A;Toshihiko Matsuo;Uchida T;Shimamura K;Ohtsuki H;松尾俊彦;松尾俊彦;松尾俊彦;松尾俊彦;松尾俊彦;松尾俊彦;Masaki Tanito
• 通讯作者: Masaki Tanito

Acceleration of Age-Related Changes in the Retina in a -Tocopherol Transfer Protein Null Mice Fed a Vitamin E-Deficient Diet.

喂食缺乏维生素 E 的饮食的生育酚转移蛋白无效小鼠视网膜中与年龄相关的变化加速。

• 发表时间: 2007
• 期刊: Investigative Ophthalmology & Vision Science 48 (1)
• 作者: Masaki;Tanito;Yasukadu;Yoshida;Sachiko;Kaidzu;Zhi-Hua;Chen;Osamu;Cynshi;Kou-ichi;Jishage;Etsuo;Niki;Akihiro;Ohira
• 通讯作者: Ohira