BIOFILM OF ORAL CANDIDA AND BIOLOGICAL INVESTIGATION ON CANDIDA-REGULATING FACTORS IN SALIVA

口腔念珠菌生物膜及唾液念珠菌调控因子的生物学研究

• 批准号: 12470397
• 负责人: OSAKI Tokio
• 金额: $ 7.3万
• 依托单位: Kochi Medical school
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470397/
• 关键词: candidiasis; lactoferrin; lactoferrin peptide; saliva; neutrophil; macrophage; reactive oxygen intermediates; NADPH; ディフェンシン

With the aim of control of fungal infections, we have examined and obtained following results.1. In patients with oral candidiasis, salivary flow rate was decreased with decreased levels of sIgA, SC, lactoferrin, and transferring.2. The Candida adhesion inhibitory activity and Candida growth inhibitory activity of saliva and Candida killing activity of salivary neutrophils were decreased in oral Candida patients.3. By again, salivary flow rate and superoxide generation of salivary neutrophils were decreased and oral Candida counts were inversely increased.4. By oral cancer treatment, Candida adhesion and Candida growth activities were increased.5. A lactoferrin peptide, Peptide 2 activated neutrophils to generate a large amount of reactive oxygen species upregulating their Candida killing activity.6. A combination of Peptide 2 and amphotericin B survived Candida-infected mice.From these results, the important role of saliva on oral Candida growth control was clarified and the control of deep candidiasis appears to succeed by applying a combination of Peptide 2 and antifungal drugs.

为了控制真菌感染，我们已经检查并获得了遵循结果。1。在口服念珠菌病的患者中，随着SIGA，SC，乳铁蛋白和转移水平降低，唾液流量降低。2。口服念珠菌患者的念珠菌粘附抑制活性和唾液和念珠菌杀伤活性的降低。3。再次，唾液流量和唾液中性粒细胞的超氧化物产生减少，口服念珠菌计数成反比。4。通过口腔癌治疗，念珠菌粘附和念珠菌生长活动增加了5。乳铁蛋白肽2肽2激活中性粒细胞，产生大量的活性氧，从而上调其念珠菌杀伤活性。6。肽2和两性霉素B的结合在感染了念珠菌的小鼠中幸存下来。从这些结果中，阐明了唾液对口服念珠菌生长控制的重要作用，并且通过施加肽2和抗真菌药物的组合，对深念珠菌的控制似乎成功了。

项目成果

Eisaku Ueta: "A novel bovine lactoferrin peptide, FKCRRWQWRM, suppressed Candida cell growth and activates neutrophils"Journal of Peptide Research. 57. 240-249 (2001)

Eisaku Ueta：“一种新型牛乳铁蛋白肽 FKCRRWQWRM 可抑制念珠菌细胞生长并激活中性粒细胞”肽研究杂志。

Eisaku Ueta: "Increase of Candida cell virulence by anticancer drugs and irradiation"Oral Microbiology and Immunology. (in press).

Eisaku Ueta：“抗癌药物和辐射增加念珠菌细胞毒力”口腔微生物学和免疫学。

Toyohiro Tanida: "Lactoferrin peptide increases the survival of Candida albicans-Inoculated mice by upregulating neutrophil and macropage functions, especially and macrophage functions, especially in combination with amphotericin B and granulocyte-macroph

Toyohiro Tanida：“乳铁蛋白肽通过上调中性粒细胞和巨噬细胞功能，特别是巨噬细胞功能，特别是与两性霉素 B 和粒细胞巨噬细胞结合使用，提高接种白色念珠菌的小鼠的存活率

Eisaku Ueta: "A novel bovine lactoferrin peptide, FKCRRWQWRM, suppressed Candida cell growth and activates neutrophils"Journal of Peptide Research. (in press).

Eisaku Ueta：“一种新型牛乳铁蛋白肽 FKCRRWQWRM 可抑制念珠菌细胞生长并激活中性粒细胞”肽研究杂志。

Eisaku Ueta: "A novel bovine lactoferrin peptide, FKCRRWQWRM, suppressed Candida cell growth and Activated neutrophils"Journal of Peptide Research. 57. 240-249 (2001)

Eisaku Ueta：“一种新型牛乳铁蛋白肽 FKCRRWQWRM 可抑制念珠菌细胞生长并激活中性粒细胞”肽研究杂志。