Functional analysis of protein phosphatase in vestibulo-cereberum induced by unitateral labyrinthectomy in rats

单侧迷路切除术诱导大鼠前庭大脑蛋白磷酸酶的功能分析

• 批准号: 09671743
• 负责人: TAKEDA Noriaki
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671743/
• 关键词: differential display; labyrinthectomy; vestibular compensation; protein phosphatase; glutamate receptor; グルタミン酸レセプター

The differential desplay method was used to identify the gene expression which was altered in the cerebellar flocculus after unilateral labyrintyectomy (UL).Total RNA from flocculi of sham-operated and labyrinthectomized rats was isolated, amplified by PCR using arbitrary primer sets and separated by electrophoresis on a polyacrylarmide gel.PCR products, of which amounts were significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the Purkinje cell layer.In labyrinthectomized rats, UL-induced nystagmus gradually disappeared within 3days after UL.These findings suggested that changes in expression of those molecules in the floccular Purkinje cells after UL is involved in vestibular compensation.

使用差异剂量方法来识别单方面迷路切除术（UL）在单方面迷路切除术（UL）后在小脑絮凝中改变的基因表达。吞噬的吞噬和迷路剪切的2骨的局部RNA通过pCR分离，并通过任意启动量在prects上分离出PRCR，并通过pcr进行了多粒子，并通过pre; significantly different in samples from labyrinthectomized rats and those from controls, were cut out of the gel and sequenced.One of the up-regulated products was the rat protein phosphatase 2A beta catalytic subunit mRNA and one of the down-regulated products was the rat glutamate receptor delta-2 subunit mRNA.Ilistochemical study of in situ hybridization showed that those molecules were localized in the在迷宫切除的大鼠中，UL诱导的眼球震颤在UL后的3天内逐渐消失。这些发现表明，UL后UL后，这些分子表达的变化与前庭补偿有关。

项目成果

Kitahara T,Takeda N,et al.: "Unilateral labyrinthectomy downregulates glutamate receptor δ-2 expression in the rat vestibulocerebellum." Mol.Brain Res.61. 170-179 (1998)

Kitahara T、Takeda N 等人：“单侧迷路切除术下调大鼠前庭小脑中谷氨酸受体 δ-2 的表达。”Mol.Brain Res.61 (1998)。

Kitahara T, Takeda N, et al.: "Changes in nitric oxide synthetase-like immunoreactivities in unipolar cells in the rat cerebellar flocculus after unilateral labyrinthectomy." Brain Res.765. 1-6 (1997)

Kitahara T、Takeda N 等人：“单侧迷路切除术后大鼠小脑絮状单极细胞中一氧化氮合成酶样免疫反应性的变化。”

北原糺、武田憲昭、他: "前庭代償における小脳プロテイン・フォスファターゼ2Aの関与・Differential Displayを用いた前庭代償関連因子の検索" 頭頚部自律神経. 11. 8-10 (1997)

Tadasu Kitahara、Noriaki Takeda 等：“前庭补偿中小脑蛋白磷酸酶 2A 的参与/使用差异显示搜索与前庭补偿相关的因素”头颈自主神经系统。

Kitahara T,Takeda N,et al.: "An implication of protein phosphatase 2A-β in rat flocculus for lesion-induced vestibular plasticity." Acta Otolaryngol.(Stockh.). 118. 685-691 (1998)

Kitahara T、Takeda N 等人：“大鼠絮状蛋白磷酸酶 2A-β 对损伤引起的前庭可塑性的影响。”Acta Otolaryngol.(Stockh.) 118. 685-691 (1998)。

武田憲昭、北原糺: "前庭代償" Equilibrium Res.56. 306-307 (1997)

Noriaki Takeda、Tadasu Kitahara：“前庭补偿”平衡 Res.56（1997）。