Inhibitory response of locus coeruleus neurons to caloric stimulation in rats

大鼠蓝斑神经元对热量刺激的抑制反应

• 批准号: 06671712
• 负责人: TAKEDA Noriaki
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671712/
• 关键词: locus coeruleus; Caloric stimulation; Vestibulo-autonomic reflex; GABA; Ventrolateral medulla; ラット; VLM; 前庭自律神経反射; カロリー刺激

We examined the effects of caloric vestibular stimulation on the neuronal activity of the locus coeruleus (LC) in urethane-anesthetized rats. The middle ear cavity was irrigated with hot (44ﾟC) or cold (30ﾟC) water through a polyethylene tube. Most neurons exhibited suppression of neuronal discharge in response to caloric stimulation. The suppression of LC neuronal discharge following caloric stimulation occured with a long latency and lasted a long period of time (about 3 min). Neither caloric stimulation of the auricle, nor irrigation of the middle ear with water at 37ﾟC,nor caloric stimulation of the middle ear after labyrinthectomy inhibited LC neuronal discharge. The caloric stimulation-induced LC neuronal inhibition was attenuated by the iontophoretic applocation of bicuculline. These findings indicate that the predominant effect of caloric stimulation on LC neuronal discharge is inhibitory, and that the caloric stimulation-induced LC ncuronal in inhibition is mediated by GABA_A receptors. We then examined the effects of lesions in the rostral vcntrolateral medulla (VML) or in the nucleus prepositus hypoglossi (PrII) on LC neuronal inhibition caused by caloric stimulation. VML lesions attenuated caloric stimulation-induced LC neuronal inhibition, but PrII lesions did not. This finding indicates that vestibular inhibition of LC neurons is mediated by VML.It is suggested that the suppressed activity of noradrenergic LC neurons is involved in the vestibulo-autonomic reflex.

我们检查了热量前庭刺激对尿烷 - 麻醉大鼠的神经元活性（LC）的神经元活性的影响。通过聚乙烯管，用热（44°C）或冷（30°C）水灌溉中耳腔。大多数神经元暴露于响应热量刺激的神经元排出。卡路里刺激后LC神经元排出的抑制是长期潜伏期发生的，并且持续了很长一段时间（约3分钟）。均未在37°C下用水灌溉的高热量刺激，也没有在迷路切除术后抑制LC神经元放电后中耳的热量刺激。卡路里刺激诱导的LC神经元抑制作用通过双核的离子疗法抑制作用。这些发现表明，热量刺激对LC神经元放电的主要作用是抑制性的，并且热量刺激诱导的LC NCURONAL在抑制中是由GABA_A接收器介导的。然后，我们检查了病变在延髓VCNTROLEFALLAL髓质（VML）中的影响，或在降临核核核酸核（PRII）对热量刺激引起的LC神经元抑制作用中的影响。 VML病变减弱了热量刺激引起的LC神经元抑制，但PRII病变没有。这一发现表明，前庭抑制LC神经元是由VML介导的。这表明Nor腺癌LC神经元的抑制活性参与了前庭自治反射。

项目成果

S.Nishiike, et al.: "Effects of caloric vestibular stimulation on prepositus hypoglossi neurons in rats." Eur.Arch.Otorhnolaryngol. 253. 52-56 (1996)

S.Nishiike 等人：“热量前庭刺激对大鼠舌前舌下神经元的影响”。

Nishiike S.et al.: "Responses of locus coeruleus neurons to caloric stimulation in rats." Acta Oto-Laryngol.Suppl.520. 105-109 (1995)

Nishiike S.et al.：“大鼠蓝斑神经元对热量刺激的反应。”

Nishiike S.et al.: "GABA ergic inhibitory response of locus coeruleus neurons to calori vestibular stimulation in rats." Brain Res.(In press). (1996)

Nishiike S.等人：“大鼠蓝斑神经元对热量前庭刺激的 GABA 能抑制反应。”

Nishiike S.,et al.: "Responses of locus coeruleus neurons to caloric stimulation in rats." Acta Oto-Laryngol. Suppl.520. 105-109 (1995)

Nishiike S. 等人：“大鼠蓝斑神经元对热量刺激的反应”。

Nishiike S. et al.: "GABAergic inhibitory response of locus coeruleus neurons to caloric vestibular stimulation in rats." Brain Res.(In press). (1996)

Nishiike S. 等人：“大鼠蓝斑神经元对热量前庭刺激的 GABA 抑制反应。”