Role of CRP-positive cerebellar climbing fiber system on the development of vestibular compensation : An animal model study.

CRP 阳性小脑攀爬纤维系统对前庭代偿发展的作用：动物模型研究。

• 批准号: 15591817
• 负责人: TAKEDA Noriaki
• 金额: $ 1.92万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591817/
• 关键词: cerecellar; animal model; corticotropin-releasing factor; vestibular cerebellum; unipolar brush cell; mossy fiber; climbing fiber; vestibular compensation

Role of CRP-positive cerebellar climbing fiber system in the development of vestibular compensationThe present immunohistochemical study demonstrated an increase in number of corticotropin-releasing factors (CRF)-positive mossy and climbing fiber terminals in the vestibulocerebellum in an ataxic mutant, rolling mouse Nagoya (RMN). Unipolar brush cells (UBCs), a class of interneurons in the vestibulocerebellum, amplifies excitatory inputs from mossy fibers. The number of calretinin (CR)-positive UBCs in the vestibulocerebellum was no different between RNM and the control mice. But, the number of CFR-positive mossy fiber terminals in closely apposition to CR-positive UBCs was 5-fold greater in RMN than in the controls. It is suggested that CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum compensate the cerebellar ataxia in RMN.An attempt was made to clarify the roles of CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum in the development of vestibular compensation. After unilateral labyrinthectomy in mice, there were no changes in number of CRF-positive mossy fiber terminals in contact with UBCs in the vestibulocerebellum. Although the number of CRF-positive climbing fiber terminals was increased in the vestibulocerebellum of RMN, there were no changes in number of CRF-positive climbing fiber terminals in the vestibulocerebellum after unilateral labyrinthectomy in mice. All these findings suggest that CRF-positive mossy and climbing fibers in the vestibulocerebellum do not play roles in the development of vestibular compensation.On the contrary, the number of CRF-positive terminals in the ipsilateral medial vestibular nucleus was decreased after unilateral labyrinthectomy in mice, suggesting that CRF affects the development of vestibular compensation in the vestibular nuclei, but not in the vestibulocerebellum.

CRP阳性小脑攀爬纤维系统在前庭补偿发展中的作用，当前的免疫组织化学研究表明，皮质激素释放因子的数量增加（CRF）阳性苔藓和攀岩纤维终末，在原始的cotibellum the hataxic mutant，croutant，croutant，crolting鼠标Nagoya（Rmnagoya）中（rmnagoya）。单极刷细胞（UBC）是前庭中脑中的一类神经元，会放大苔藓纤维的兴奋性输入。 RNM和对照小鼠之间的钙蛋白素（CR）阳性UBC的数量没有什么不同。但是，在RMN中，与CR阳性UBC的CFR阳性苔藓纤维端子的数量相比，在CR阳性的UBC上的数量比对照组大5倍。建议与crf阳性的苔藓纤维终端与crtibulocerebellum接触的CRF阳性纤维末端补偿了RMN中的小脑共济失调。在小鼠的单侧迷宫切除术后，与前庭脑中UBC接触的CRF阳性苔藓纤维末端的数量没有变化。尽管在RMN的前庭脑脑中，CRF阳性攀岩纤维末端的数量增加了，但小鼠单侧迷路切除术后前庭纤维中的CRF阳性攀爬纤维末端的数量没有变化。 All these findings suggest that CRF-positive mossy and climbing fibers in the vestibulocerebellum do not play roles in the development of vestibular compensation.On the contrary, the number of CRF-positive terminals in the ipsilateral medial vestibular nucleus was decreased after unilateral labyrinthectomy in mice, suggesting that CRF affects the development of vestibular compensation in the vestibular nuclei,但不在前庭脑中。

项目成果

Abnornal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal

共济失调突变小鼠、滚动小鼠Nagoya和稀致死小鼠小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: K.Sawada;M.Ando;H.Sakata-haga;X.Z.Sun;Y.G.Jeong;S.Hisano;N.Takeda;Y.Fukui;Kazuhiko Sawada
• 通讯作者: Kazuhiko Sawada

Abnormal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal

共济失调突变小鼠、滚动小鼠Nagoya和稀致死小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: Sawada;K.;Ando;M.;Sakata-Haga;H.;Sun;X-Z.;Jeong;Y.-G.;Hisano;S.;Takeda;N.;Fukui;Y.
• 通讯作者: Y.

安藤正裕, 澤田和彦, 武田憲昭, 他: "ローリングマウス小脳におけるカルレチニン陽性unipolar brush cellにおけるCRF苔状線維終末"頭頸部自律神経. 18. 53-55 (2004)

Masahiro Ando、Kazuhiko Sawada、Noriaki Takeda 等：“滚动小鼠小脑中钙视网膜蛋白阳性单极刷状细胞中的 CRF 苔藓纤维末端”头颈自主神经学。

Abnornal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rollimg mouse Nagoya and dilute-lethal

共济失调突变小鼠、rollimg小鼠Nagoya和稀致死小鼠小脑浦肯野细胞酪氨酸羟化酶异常表达，不伴有丝氨酸40磷酸化

• 发表时间: 2004
• 期刊: Congenital Anomalies 44
• 作者: Kazuhiko Sawada