Application of Fourier transformation-linear ion trap mass spectrometers to forensic medicine

傅里叶变换线性离子阱质谱仪在法医学中的应用

• 批准号: 23390183
• 负责人: ISHII Akira
• 金额: $ 10.32万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390183/
• 关键词: 法中毒学; 質量分析; オービトラップ; 飛行時間型質量分析計; 自然毒; アマニチン; 合成カンナビノイド; ミフェプリストン; タンデム質量分析; 飛行時間型; 代謝物; UPLC-MS-MS; Orbitrap; Q-TOF; 法医学; 中毒学; 分析化学

The purpose of the project is to develop application methods for identifying and quantitating various drugs and toxins using a Fourier transformation-linear ion trap mass spectrometer, shared in the Faculty. However, under the available LC conditions optimized to proteomic analysis, sufficient separation and reproducibility were not obtained. We thus purchased a quadrupole-time of flight mass spectrometer (Q-TOFMS); further experiments were rendered using this (liquid chromatography) LC-Q-TOFMS system.Using the LC-Q-TOFMS system, amanitins in urine samples were sufficiently separated; the calibration curves showed good linearity in the range of 1 - 5,000 ng/ml. This system has enabled sensitive detection and identification of various synthetic cannabinoids and mifepristone, and their metabolites with high sensitivity. We are now constructing a screening system of 53 natural toxins. Hence, LC-Q-TOFMS will be a useful tool in analyzing various compounds in forensic toxicology.

该项目的目的是开发使用学院共享的傅里叶变换线性离子阱质谱仪来识别和定量各种药物和毒素的应用方法。 然而，在针对蛋白质组分析优化的现有 LC 条件下，并未获得足够的分离和重现性。 因此，我们购买了四极杆飞行时间质谱仪（Q-TOFMS）；使用该（液相色谱）LC-Q-TOFMS系统进行了进一步的实验。使用LC-Q-TOFMS系统，尿样中的鹅膏蕈碱得到充分分离；校准曲线在 1 - 5,000 ng/ml 范围内表现出良好的线性。 该系统能够以高灵敏度灵敏地检测和鉴定各种合成大麻素和米非司酮及其代谢物。 我们现在正在构建53种天然毒素的筛查系统。 因此，LC-Q-TOFMS 将成为法医毒理学中分析各种化合物的有用工具。

项目成果

Recently abused synthetic cathinones, α-pyrrolidinophenone derivatives : a review of their pharmacology, acute toxicity, and metabolism

最近滥用的合成卡西酮、α-吡咯烷酮衍生物：对其药理学、急性毒性和代谢的综述

• 发表时间: 2014
• 影响因子: 2.2
• 作者: Zaitsu K; Katagi M; Tsuchihasi H; Ishii A
• 通讯作者: Ishii A

Metabolism of the newly encountered designer drug α-pyrrolidinovalerophenone in humans: identification and quantitation of urinary metabolites.

新遇到的设计药物 α-吡咯烷戊苯酮在人体中的代谢：尿代谢物的鉴定和定量。

• DOI: 10.1007/s11419-013-0202-9
• 发表时间: 2014
• 影响因子: 2.2
• 作者: Noriaki Shima; Munehiro Katagi; Hiroe Kamata; Shuntaro Matsuta; Keiko Sasaki; Tohru Kamata; Hiroshi Nishioka; Akihiro Miki; Michiaki Tatsuno; Kei Zaitsu; Akira Ishii; Takako Sato; Hitoshi Tsuchihashi; Koichi Suzuki
• 通讯作者: Koichi Suzuki

Prevalence of new designer drugs and their legal status in Japan

新设计药物的流行及其在日本的法律地位

• 发表时间: 2013
• 影响因子: 0.3
• 作者: Kikura

Two new-type cannabimimetic quinolinyl carboxylates, QUPIC and QUCHIC, two new cannnabimimetic carboxamide derivatives, ADB-FUBINACA and ADBICA, and five synthetic cannabinoids detected with a thiophene derivative, α-PVT, and an opioid receptor agonist, A

两种新型大麻模拟喹啉基羧酸酯，QUPIC 和 QUCHIC，两种新型大麻模拟甲酰胺衍生物，ADB-FUBINACA 和 ADBICA，以及用噻吩衍生物 α-PVT 和阿片受体激动剂 A 检测到的五种合成大麻素

• DOI: 10.1007/s11419-013-0182-9
• 发表时间: 2013
• 影响因子: 2.2
• 作者: N. Uchiyama; S. Matsuda; M. Kawamura; R. Kikura
• 通讯作者: R. Kikura

Identification and quantitation of JWH-213, a cannabimimetic indole, as a designer drug in an herbal product.

JWH-213（一种大麻模拟吲哚）作为草药产品中的设计药物的鉴定和定量。

• DOI: 10.1007/s11419-012-0161-6
• 发表时间: 2013
• 影响因子: 2.2
• 作者: K. Takahashi; N. Uchiyama; T. Fukiwake; T. Hasegawa; M. Saijou; Y. Motoki; R. Kikura
• 通讯作者: R. Kikura