New strategy based on the organogenesis signal control for periodontal disease

基于器官发生信号控制的牙周病新策略

• 批准号: 21390553
• 负责人: IZUMI Yuichi
• 金额: $ 11.56万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390553/
• 关键词: 歯周免疫機能学; Wnt5a; 歯周炎; 単球; P. gingivalis LPS; P.gingivalis LPS; STAT1; IFN-γ

Wnt5a mRNA expression was up-regulated in chronic periodontitis tissue as compared to healthy control tissue. P. gingivalis LPS induced Wnt5a mRNA in the human monocytic cell line THP-1 with a peak at 4 hrs after stimulation. P. gingivalis LPS induced higher up-regulation of Wnt5a mRNA than E. coli LPS. The LPS receptors TLR2 and TLR4 were equally expressed on the surface of THP-1 cells. P. gingivalis LPS induced IkBa degradation and was able to increase the NF-kB binding activity to DNA. P. gingivalis LPS-induced Wnt5a expression was inhibited by NF-kB inhibitors, suggesting NF-kB involvement. Furthermore, IFN-g synergistically enhanced the P. gingivalis LPS-induced production of Wnt5a. Pharmacological investigation and siRNA experiments showed that STAT1 was important for P. gingivalis LPS-induced Wnt5a expression. These results suggest that the modulation of Wnt5a expression by P. gingivalis may play an important role in the periodontal inflammatory process and serve a target for the development of new therapies.

与健康对照组织相比，慢性牙周炎组织中 Wnt5a mRNA 表达上调。牙龈卟啉单胞菌 LPS 在人单核细胞系 THP-1 中诱导 Wnt5a mRNA，并在刺激后 4 小时达到峰值。牙龈卟啉单胞菌 LPS 诱导 Wnt5a mRNA 的上调程度高于大肠杆菌 LPS。 LPS受体TLR2和TLR4在THP-1细胞表面同等表达。牙龈卟啉单胞菌 LPS 诱导 IkBa 降解并能够增加 NF-kB 与 DNA 的结合活性。牙龈卟啉单胞菌 LPS 诱导的 Wnt5a 表达被 NF-kB 抑制剂抑制，表明 NF-kB 参与其中。此外，IFN-g 协同增强牙龈卟啉单胞菌 LPS 诱导的 Wnt5a 产生。药理学研究和siRNA实验表明STAT1对于牙龈卟啉单胞菌LPS诱导的Wnt5a表达很重要。这些结果表明牙龈卟啉单胞菌对Wnt5a表达的调节可能在牙周炎症过程中发挥重要作用，并成为开发新疗法的靶点。

项目成果

Induction of Wnt5a in THP-1 cells by periodontopathic bacteria

牙周病细菌在 THP-1 细胞中诱导 Wnt5a

• 发表时间: 2010
• 作者: Nanbara H; Kobayashi H; Izumi Y; et al
• 通讯作者: et al

Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods.

通过生长和酶消化培养方法获得的牙周膜细胞的特征比较。

• DOI: 10.1016/j.archoralbio.2010.10.013
• 发表时间: 2011-04-01
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: Keiko Tanaka;K. Iwasaki;K. Feghali;M. Komaki;I. Ishikawa;Y. Izumi
• 通讯作者: Y. Izumi

Hydrogen sulfide causes apoptosis in human pulp stem cells.

硫化氢会导致人牙髓干细胞凋亡。

• DOI: 10.1016/j.joen.2011.01.017
• 发表时间: 2011-04-01
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Chie Kobayashi;K. Yaegaki;B. Calenic;N. Ishkitiev;T. Imai;Hisataka Ii;I. Aoyama;H. Kobayashi;Y. Izumi;M. Haapasalo
• 通讯作者: M. Haapasalo

Structural differences in the osteocyte networ between the calvaria and long bone revealed by three-dimensional fluorescence morphometry, possibly reflecting dstinct mechano-adaptations and sensitivities

三维荧光形态测量显示颅骨和长骨之间骨细胞网络的结构差异，可能反映了不同的机械适应和敏感性

• DOI: 10.1016/j.bbrc.2011.12.031
• 发表时间: 2011
• 作者: Himeno

Functions of PI3K and MAPK on Wnt5a expression in THP-1

PI3K和MAPK对THP-1中Wnt5a表达的作用

• 发表时间: 2010
• 作者: Nanbara H; Kobayashi H; Izumi Y; et al
• 通讯作者: et al