Electrophysiological analysis for pain response by the lateral bed nucleus stria terminalis

侧床终纹核疼痛反应的电生理分析

• 批准号: 24790583
• 负责人: HAGIWARA Hiroko
• 金额: $ 2.91万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790583/
• 关键词: ホルマリンテスト; マウス; 分界条床核外側部; CRH; GFP; パッチクランプ; スライス; 性差; CRHニューロン; 情動; 痛み; ホルマリン; ドーパミンニューロン

We discovered that the lateral bed nucleus stria terminalis (BST) is involved in the pain response during formalin test. Conversely, the function of the lateral BST influences the pain response by the formalin. This concept indicates the emotion and pain is related to each other. CRH neurons are expressed the brain area such as the periventricular hypothalamus associated with stress and the central amygdala related to outflow of the emotional response. Thus, CRH neurons in the lateral BST may be involved in emotion side during the formalin test. To this end we must label CRH neurons as fluorescence since we will analyze by patch-clamp technique. So we are now generating transgenic mouse which CRH neurons have bright fluorescence and useful for electrical analyse.

我们发现侧床终纹核（BST）参与福尔马林试验期间的疼痛反应。相反，外侧 BST 的功能影响福尔马林的疼痛反应。这个概念表明情绪和痛苦是相互关联的。 CRH 神经元在大脑区域表达，例如与压力相关的室周下丘脑和与情绪反应流出相关的中央杏仁核。因此，外侧 BST 中的 CRH 神经元可能在福尔马林测试过程中参与情绪侧。为此，我们必须将 CRH 神经元标记为荧光，因为我们将通过膜片钳技术进行分析。因此，我们现在正在培育转基因小鼠，其 CRH 神经元具有明亮的荧光，可用于电分析。

项目成果

Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding

• DOI: 10.1097/wnr.0000000000000172
• 发表时间: 2014-07-09
• 期刊: NEUROREPORT
• 影响因子: 1.7
• 作者: Fukushima, Atsushi;Hagiwara, Hiroko;Funabashi, Toshiya
• 通讯作者: Funabashi, Toshiya

Sex-specific differences in pain response by dopamine in the bed nucleus of the stria terminalis in rats

• DOI: 10.1097/wnr.0b013e32835d8540
• 发表时间: 2013-03-06
• 期刊: NEUROREPORT
• 影响因子: 1.7
• 作者: Hagiwara, Hiroko;Funabashi, Toshiya;Kimura, Fukuko
• 通讯作者: Kimura, Fukuko