Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene that contributes to activation of host innate immunity

益生菌干酪乳杆菌的转座子诱变鉴定出 asnH，一种有助于激活宿主先天免疫的天冬酰胺合成酶基因

• 批准号: 23790485
• 负责人: ITO Masahiro
• 金额: $ 2.83万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790485/
• 关键词: プロバイオティクス; 自然免疫; 菌体表層構造; 自然免疫賦活化

We demonstrated that AsnH exhibits asparagine synthetase activity when the lysozyme-treated cell wall extract of L. casei was added as substrate. L. caseicarrying mutated asnH lacked its characteristic thick and rigid cell wall structure, indicating that AsnH of L. casei is involved in cell wall biosynthesis. These results indicate that asnH is required for bacterial cell wall biosynthesis and is involved in the probiotic activity of L. casei ATCC 27139.

我们证明，当添加经溶菌酶处理的干酪乳杆菌细胞壁提取物作为底物时，AsnH 表现出天冬酰胺合成酶活性。携带突变asnH的干酪乳杆菌缺乏其特有的厚且刚性的细胞壁结构，表明干酪乳杆菌的AsnH参与细胞壁生物合成。这些结果表明 asnH 是细菌细胞壁生物合成所必需的，并且参与干酪乳杆菌 ATCC 27139 的益生菌活性。

项目成果

Asparagine Synthetase Plays an Important Role in Enhancement of Host Innate Immunity by Probiotic Lactobacillus casei

天冬酰胺合成酶在益生菌干酪乳杆菌增强宿主先天免疫中发挥重要作用

• 发表时间: 2013
• 作者: Masahiro Ito;Yun-Gi Kim;Hirokazu Tsuji;Takuya Takahashi;Mayumi Kiwaki;Koji Nomoto;Hirofumi Danbara;Nobuhiko Okada
• 通讯作者: Nobuhiko Okada

Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene that contributes to activation of host innate immunity

益生菌干酪乳杆菌的转座子诱变鉴定出 asnH，一种有助于激活宿主先天免疫的天冬酰胺合成酶基因

• 发表时间: 2013
• 作者: 大岡唯祐;勢戸和子;磯部順子;山口敬治;瀬戸順次;小椋義俊;林哲也;小川道永;日吉大貴;小椋義俊, 林哲也;Masahiro Ito
• 通讯作者: Masahiro Ito