Evaluation of cell damage and allergy-induced mechanism by minute substance eluted from dental bio-materials

通过牙科生物材料中洗脱的微小物质评估细胞损伤和过敏诱发机制

• 批准号: 15390597
• 负责人: TAIRA Masayuki
• 金额: $ 7.04万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390597/
• 关键词: Nickel ion; Titanium ion; Macrophage; Cell viability; Apoptosis; Superoxide dismutase; Inflammatory cytokine; DNA microarray; リポポリサッチャライド; TNF-α; Niイオン; RAW264; DNA損傷; Ti粉末; 貪食; 活性酸素; ハプテン形成; マウスマクロファージ; NO産生量; PIXE; DNAマイクロアレー; 解毒系蛋白

(1)With increasing nickel ion concentrations, cell viability of macrophage-like RAW264 cells declined, and nitric oxide production of LPS-stimulated RAW 264 cells also declined. DNA microarray analyses showed that nickel ions caused cell cycle arrest, induction of apoptosis, production of inflammatory cytokines (IL-1α、IL-1α、IL-6、TNF) and increased oxidative stress, which lead to cellular damage and systematic body damage such as allergy and cancer.(2)Cell viability of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions declined to about 55%, compared to that of control cells without titanium ions. SOD activities and TNF-α production of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions doubled, compared to those of the control cells without titanium ions. It was speculated that phagocytosis of titanium-protein complex by macrophage caused inflammatory cellular reactions.(3)Elution of 10μm titanium particles into distilled water, MEM solution and lactic acid for 15 days was found to be negligible (0), 0.01 ppm and 9 ppm, respectively. Cell viability and NO production of macrophage-like RAW264 cells that phagocytized 10μm titanium particles were 89.4% and 91.6%, respectively. This means that macropage's cellular damage caisued by phagocytosis of 10μm titanium particles was small and tolerable.

(1)随着镍离子浓度的增加，巨噬细胞样RAW264细胞的细胞活力下降，LPS刺激的RAW 264细胞的一氧化氮产生也下降，DNA微阵列分析表明镍离子导致细胞周期停滞、诱导细胞凋亡、产生。炎症细胞因子（IL-1α、IL-1α、IL-6、TNF）的减少和氧化应激的增加，从而导致细胞损伤和系统性身体损伤，例如过敏(2)与不含钛离子的对照细胞相比，在含有1ppm钛离子的培养基中培养的巨噬细胞样RAW264细胞的细胞活力下降至约55%。与不含钛离子的对照细胞相比，在含有1ppm钛离子的培养基中培养的细胞的吞噬作用增加了一倍。 (3)将10μm钛颗粒洗脱到蒸馏水、MEM溶液和乳酸中15天，结果发现分别可以忽略不计(0)、0.01 ppm和9 ppm。吞噬10μm钛颗粒的巨噬细胞样RAW264细胞的活力和NO产生率为89.4%分别为91.6%，这意味着macropage吞噬10μm钛颗粒造成的细胞损伤较小且可以忍受。

项目成果

DNA microarray analyses of gene expression in human mesenchymal stem cells cultured in osteogenic differentiation medium for 14 days

DNA微阵列分析在成骨分化培养基中培养14天的人间充质干细胞的基因表达

• 发表时间: 2005
• 作者: Taira; M.; Chosa N.; Sasaki; K.; Saitoh; S.; Nezu; T.; Sato; N.; Araki Y.
• 通讯作者: Araki Y.

Effect of Ni2+ ions on cell viability and NO production of murine periotoneal exudate cells (macrophages) with and without lipopolysaccharide stimulation

Ni2 离子对有和没有脂多糖刺激的小鼠腹膜渗出细胞（巨噬细胞）的细胞活力和 NO 产生的影响

• 发表时间: 2005
• 作者: Taira; M.; Sasaki; M.; Yamaura
• 通讯作者: Yamaura

Taira, M., Chosa, N., Sasaki K., Saitoh, S., Sato, N., Takahashi, J., Araki, Y.: "Eeffects of the medium, serum and dexamethasone on the cell proliferation and alkaline phosphatase activity of twice-passaged SD rats' bone marrow stromal cells"Journal of O

Taira, M., Chosa, N., Sasaki K., Saitoh, S., Sato, N., Takahashi, J., Araki, Y.：“培养基、血清和地塞米松对细胞增殖和碱性磷酸酶的影响

Effects of Ni^ ions on cell viability and NO production of murine periotoneal exudate cells (macrophages) with and without Iipopolysaccharide stimulation.

Ni 2 离子对有和没有脂多糖刺激的小鼠腹膜渗出细胞(巨噬细胞)的细胞活力和NO产生的影响。

• 发表时间: 2005
• 作者: Taira; M.; Sasaki; M.; Yamaura
• 通讯作者: Yamaura

Microarray analysis of the cell cycle-related gene expression from a RAW264 murine macrophage cell line in response to lipopolysaccharide

RAW264 鼠巨噬细胞系响应脂多糖的细胞周期相关基因表达的微阵列分析

• 发表时间: 2005
• 作者: Taira; M.; Sasaki; M.; Kimura; S.; Araki; Y.
• 通讯作者: Y.