Development of a Microdevice for Photoelectrical Stimulation of Cells

细胞光电刺激微型装置的开发

• 批准号: 10263886
• 负责人: Jingjiao Guan
• 金额: $ 7.1万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10263886
• 关键词: Acids; Alkanesulfonates; Allylamine; Astrocytes; Atomic Force Microscopy; Behavior; Binding; Biological Assay; Bovine Serum Albumin; Brain; Butyric Acids; Calcium ion; Caliber; Cell Differentiation process; Cell Proliferation; Cell membrane; Cell surface; Cells; Cultured Cells; Development; Devices; Dimensions; Dyes; Electric Stimulation; Electrons; Engineering; Ensure; Esters; Fluorescence; Foundations; Generations; Glutaral; Gold; Hippocampus (Brain); Human; Implanted Electrodes; In Vitro; Individual; Ion Channel; Light; Lighting; Literature; Measures; Microprocessor; Microscopy; Modeling; Mus; Nerve Tissue; Neurons; Optics; Organoids; Pattern; Performance; Physiologic pulse; Polymers; Protocols documentation; Research; Resolution; Scanning Electron Microscopy; Shapes; Signal Transduction; Structure; Styrenes; Surface; Surface Properties; Techniques; Thick; Time; Tissue Engineering; Tissues; Titanium; base; cell type; design; effective therapy; flexibility; microdevice; nervous system disorder; neuroblastoma cell; neuroregulation; novel; novel therapeutics; programs; titanium dioxide; tool; voltage

PROJECT SUMMARY/ABSTRACT Photoelectrical stimulation of cells is an emerging technique with tremendous potential for expanding the toolset for tissue engineering as well as enabling new therapies for treating neurological diseases, but its potential applications are limited by the large size of the existing photoelectrical-stimulation devices. The objective of this proposed study is to develop a novel device for photoelectrical stimulation. The device, which is termed microdevice, is featured by a sub-10 µm size and disk-like shape, a well-defined layered structure for converting light to electrical signals via the photovoltaic mechanism, and engineerable surface properties. These features potentially allow the microdevices to bind to individual cells and modulate their behaviors through photoelectrical stimulation. The specific aims are to (1) establish a protocol for fabricating the designed microdevices, and (2) evaluate the performance of the microdevices in photoelectrical stimulation of cells. Successful completion of the proposed research will validate feasibility of the microdevice and lay a foundation for its further development into a viable tool for tissue engineering research and neuromodulation therapy.

项目概要/摘要 细胞的光电刺激是一种新兴技术，具有扩展细胞的巨大潜力 组织工程工具集以及治疗神经系统疾病的新疗法，但它 现有光电刺激装置的大尺寸限制了潜在的应用。 这项研究的目的是开发一种新型光电刺激装置。 称为微型器件，其特点是尺寸小于 10 µm，形状呈盘状，是一种明确的层状结构，可用于 通过光伏机制和可设计的表面特性将光转换为电信号。 这些功能可能允许微型设备与单个细胞结合并通过以下方式调节它们的行为 具体目标是（1）建立用于制造设计的协议。 微型器件，(2)评估微型器件在细胞光电刺激中的性能。 拟议研究的成功完成将为验证微型器件的可行性并奠定基础 因其进一步发展成为组织工程研究和神经调节治疗的可行工具。