Studies on dual-functioning of bio-absorbable scaffold materials and cells for the rehabilitation of oral tissues

生物可吸收支架材料与细胞双重功能用于口腔组织康复的研究

基本信息

批准号：
12671880
负责人：
TAIRA Masayuki
金额：
$ 2.3万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The findings obtained are described in the following four categories.(1) Studies on the preparation of bio-absorbable scaffold materials : We succeeded in producing inter-connected porous sponges by freeze-drying from natural materials (collagen and gelatin) and synthetic materials (alginate and poly(lactide-caprolacton)).(2) Studies on the cell culture : We investigated the effect of medium type (alfa-ME or DME), serum amount, dexamethasone induction and the growth factors (e.g. TGF-beta) on the proliferation and differentiation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells. The former cells required the addition of vitamin C and the serum amount of more than 10% for the cell proliferation. The dexamethasone and the growth factors appeared to accelerate the osteogenic differentiation.(3) Studies on the duplex-functioning of the cells and scaffold materials : we successfully cultured the rat bone marrow stromal cells in the collagen gel frame-worked by poly (lactide- caprolacton) sponge. We examined the degree of osteogenic differentiation of rat bone marrow stromal cells cultured in the collagen gel. With the DME medium supplemented with vitamin C, the cells could differentiate into the pre-osteoblasts with their phenotype characterized by the appearance of mRNA for osteopontin. The cells in this stage appear to contribute to the rehabilitation of lost oral hard tissues in the future.(4) Studies on the tissue reaction against the scaffold materials : We implanted prepared scaffold materials (collagen gel, collagen sponge, poly (lactide-caprolacton) sponge, and gelatin hydro-gel) in the back tissue of SD rats, and evaluated the histo-pathological reaction against these scaffold materials. All scaffold materials were mildly absorbed by the host tissue without any strong pathological reaction.

所获得的发现分为以下四类。（1）生物可吸收支架材料的制备研究：我们成功地利用天然材料（胶原蛋白和明胶）和合成材料（胶原蛋白和明胶）冷冻干燥制备了互连的多孔海绵。 (2) 细胞培养研究：我们研究了培养基类型（alfa-ME 或 DME）、血清量、地塞米松诱导和生长因子的影响(例如TGF-β)对大鼠骨髓基质细胞和成骨细胞MC3T3-E1细胞增殖和分化的影响。前者的细胞需要添加维生素C，且血清量超过10%才能细胞增殖。地塞米松和生长因子似乎加速了成骨分化。(3)细胞和支架材料双重功能的研究：我们成功地在聚丙交酯胶原凝胶框架中培养了大鼠骨髓基质细胞。己内酯）海绵。我们检查了在胶原凝胶中培养的大鼠骨髓基质细胞的成骨分化程度。在添加了维生素 C 的 DME 培养基中，细胞可以分化为前成骨细胞，其表型以骨桥蛋白 mRNA 的出现为特征。此阶段的细胞似乎有助于将来丢失的口腔硬组织的康复。（4）对支架材料的组织反应的研究：我们植入准备好的支架材料（胶原凝胶，胶原海绵，聚（丙交酯-己内酯））海绵和明胶水凝胶）在SD大鼠的背部组织中，并评估对这些支架材料的组织病理学反应。所有支架材料均被宿主组织轻度吸收，无强烈病理反应。