Assessment of the efficacy of collagen-coated-bead columns for antiplatelet therapy in clinical settings

评估临床环境中胶原蛋白涂层珠柱抗血小板治疗的功效

• 批准号: 21790532
• 负责人: KANEKO Makoto
• 金额: $ 2.33万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790532/
• 关键词: 血小板機能検査; 抗血小板薬; コラーゲンビーズカラム; 薬効モニタリング; 抗血小板剤

In this study, we evaluated the utility of 2 types of collagen-coated-bead columns in the assessment of platelet function associated with the efficacy of antiplatelet drug therapy and prediction of bleeding. In particular, platelet activity was measured to diagnose whether patients with thrombotic disease were drug resistant and to assess patients who were or were not administered antiplatelet agents before endoscopic biopsy.Large variation in the platelet retention rate among healthy individuals was observed using the collagen-coated-bead column; this may reflect individual differences but not methodological variability. Overlapping of the retention rates of patients who were and were not administered the antiplatelet reagents may limit the establishment of reference intervals for platelet function in the present assay system. However, the platelet retention rate may reflect individual variability in response to the antiplatelet drugs and may be useful for monitoring the efficacy of antiplatelet therapy in the same individual. Thus, it is important to evaluate platelet function before and after medication for monitoring the effects of antiplatelet drugs. We also evaluated platelet function by using light-transmission platelet aggregometer and whole-blood aggregometer with a screen filtration pressure method. Platelet function assays are not interchangeable and may vary on the basis of the assay used. These findings suggest that the platelet function assay needs to be used in suitable methods and to be evaluated for additional verification of its clinical utility.

在这项研究中，我们评估了两种类型的胶原蛋白涂层孔柱的效用，以评估与抗血小板药物治疗和出血预测相关的血小板功能。特别是，测量血小板活性以诊断血栓性疾病患者是否具有抗药性，并评估内窥镜活检之前或未给予抗血小板药物的患者。使用胶原蛋白涂层螺柱观察到健康个体中血小板保留率的差异；这可能反映出个体差异，但不能反映方法上的可变性。抗血小板试剂的保留率的重叠可能会限制在本测定系统中血小板功能的参考间隔的建立。但是，血小板的保留率可能反映了对抗血小板药物的响应的个体变异性，并且可能有助于监测抗血小板疗法在同一个体中的疗效。因此，重要的是在药物前后评估血小板功能以监测抗血小板药物的作用。我们还通过使用屏幕过滤压力法的光递送血小板聚合计和全血聚合表来评估血小板功能。血小板功能测定不可互换，并且可能会根据所使用的测定而有所不同。这些发现表明，血小板功能分析需要用于合适的方法，并进行评估以额外验证其临床实用性。

项目成果

血小板・凝固検査

血小板/凝血测试

• 发表时间: 2011
• 作者: 金子誠;矢冨裕;金子誠
• 通讯作者: 金子誠

血液領域におけるピットフォール 血小板凝集能検査法の標準化 現状と問題点

血液领域的陷阱 血小板聚集检测方法标准化现状及问题

• 发表时间: 2009
• 期刊: 日本臨床検査自動化学会会誌
• 作者: 金子誠;佐藤金夫;尾崎由基男
• 通讯作者: 尾崎由基男

Evaluation of safety of endoscopic biopsy without cessation of antithrombotic agents in Japan

• DOI: 10.1007/s00535-012-0538-7
• 发表时间: 2012-07-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Ono, Satoshi;Fujishiro, Mitsuhiro;Koike, Kazuhiko
• 通讯作者: Koike, Kazuhiko

抗血小板薬に対する反応多様性

抗血小板药物反应的多样性

• 发表时间: 2011
• 期刊: 日本検査血液学会雑誌
• 作者: Ono S;Fujishiro M;Kodashima S;Takahashi Y;Minatsuki C;Mikami-Matsuda R;Asada-Hirayama I;Konno-Shimizu M;Tsuji Y;Mochizuki S;Niimi K;Yamamichi N;Kaneko M;Yatomi Y;Koike K;金子誠
• 通讯作者: 金子誠

血小板関連疾患 血小板機能異常症の診断と対応

血小板相关疾病 血小板功能障碍的诊断与治疗

• 发表时间: 2009
• 期刊: 日本血栓止血学会誌
• 作者: 金子誠;矢冨裕
• 通讯作者: 矢冨裕