The cytotoxic mechanism of Vibiro parahaemolytiucs thermostable direct hemolysin and its application to a probe for lipid rafts

副溶血弧菌耐热直接溶血素的细胞毒机制及其在脂筏探针中的应用

• 批准号: 21790420
• 负责人: MATSUDA Shigeaki
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790420/
• 关键词: 腸炎ビブリオ; 耐熱性溶血毒; 細胞傷害性; 脂質ラフト

Vibrio parahaemolyticus thermostable direct hemolysin (TDH) is associated with lipid rafts of cultured cells. This study addressed whether internalization of TDH correlates with its cytotoxicity. Inhibition of endocytosis did not affect TDH cytotoxicity for HeLa cells, indicating that internalization of TDH is not involved in its cytotoxicity. In contrast to cytotoxicity, hemolysis by TDH was independent of lipid rafts, suggesting that the mechanism of cytotoxicity by TDH appears to differ from that of hemolysis by TDH. Fluorescent dye-conjugated TDH was also constructed. This conjugate retained the cytotoxity and was associated with lipid rafts.

副溶血弧菌耐热直接溶血素 (TDH) 与培养细胞的脂筏有关。本研究探讨了 TDH 的内化是否与其细胞毒性相关。内吞作用的抑制并不影响 TDH 对 HeLa 细胞的细胞毒性，表明 TDH 的内化不参与其细胞毒性。与细胞毒性相反，TDH 的溶血与脂筏无关，这表明 TDH 的细胞毒性机制似乎与 TDH 的溶血不同。还构建了荧光染料缀合的 TDH。该缀合物保留了细胞毒性并与脂筏相关。

项目成果

Cytotoxic effect of Vibrio parahaemoly ticus thermostable direct hemolysin is dependent on lipid rafts.

副溶血弧菌耐热直接溶血素的细胞毒性作用依赖于脂筏。

• 发表时间: 2010
• 作者: Shigeaki Matsuda;Toshio Kodama;Natsumi Okada;Takeshi Honda;Tetsuya Iida
• 通讯作者: Tetsuya Iida

Identification and Characterization of a Novel Type III Secretion System in trh-Positive Vibrio parahaemolyticus Strain TH3996 Reveal Genetic Lineage and Diversity of Pathogenic Machinery beyond the Species Level

• DOI: 10.1128/iai.01184-08
• 发表时间: 2009-02-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Okada, Natsumi;Iida, Tetsuya;Honda, Takeshi
• 通讯作者: Honda, Takeshi

Cytotoxic effect of Vibrio parahaemolyticus thermostable direct bemolysin is dependent on lipid rafts

副溶血弧菌耐热直接贝莫溶素的细胞毒性作用依赖于脂筏

• 发表时间: 2010
• 作者: S.Matsuda;T.Kodama;N.Okada;T.Honda;T.Iida
• 通讯作者: T.Iida

Association of Vibrio parahaemolyticus Thermostable Direct Hemolysin with Lipid Rafts Is Essential for Cytotoxicity but Not Hemolytic Activity

• DOI: 10.1128/iai.00946-09
• 发表时间: 2010-02-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Matsuda, Shigeaki;Kodama, Toshio;Iida, Tetsuya
• 通讯作者: Iida, Tetsuya