Development of a nonhuman primate model for dengue virus infection

开发登革热病毒感染的非人灵长类动物模型

• 批准号: 21780274
• 负责人: KOBAYASHI Takeshi
• 金额: $ 2.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780274/
• 关键词: デングウイルス; サルモデル

To develop a nonhuman primate model for dengue virus (DV) infection, rhesus monkeys were inoculated with DV types 1 and 2. The DV types 1 and 2 induced viremia with duration from 2 to 3 days and a mean peak titer of 4×10^3 RNA copies/ml but infected monkeys showed no sign of dengue diseases even after inoculation of high virus doses. DV type 2 effectively replicates in MDM cells derived from bonnet monkeys compared to those derived from rhesus monkeys. These results suggest that bonnet monkey may be a useful model to study dengue disease.To improve a reverse genetics for tick-borne encephalitis virus (TBEV) which belongs to the same member of the Flavivirus genus as DV, we have constructed a plasmid-based infectious clone encoding full-length TBEV cDNA under control of the minimum cytomegalovirus promoter. Transfection of cells with the plasmid resulted in the production of infectious TBEV at high levels. Furthermore, we have developed homologous recombination-based reverse genetics for TBEV and DV. The reverse genetics approaches described here can be exploited for studies of DV replication and pathogenesis as powerful tools.

为了开发登革热病毒（DV）的非人类灵长类动物模型，恒河猴被DV型1和2接种。DV型1和2诱导的EMIA，持续时间为2至3天，平均峰值滴度为4×10^ 3 RNA副本/ML，但Inngue疾病接种了高病毒剂量的MDM细胞。 - 脑炎病毒（TBEV）黄病毒属作为DV，我们构建了一个基于质粒的感染性克隆，该克隆在控制最小的胞质病毒启动子的控制下结构了全长的TBEV cDNA。 DV。在此描述的反向遗传学被利用，以研究DV复制和发病机理作为屁股强大的工具。

项目成果

Identification of Reovirus Cell-tropism Determinants in Replication Protein mu2

复制蛋白 mu2 中呼肠孤病毒向细胞性决定因素的鉴定

• 发表时间: 2009
• 作者: Laura S.Ooms;Takeshi Kobavashi;Terence S.Dermody;James D.Chappell.
• 通讯作者: James D.Chappell.

The Reovirus m2 Protein Inhibits Interferon Signaling Through a Novel Mechanism Involving Nuclear Accumulation of Interferon Regulatory Factor-9

呼肠孤病毒 m2 蛋白通过涉及干扰素调节因子 9 核积聚的新机制抑制干扰素信号传导

• 发表时间: 2009
• 期刊: Journal of Virology 83
• 作者: Zurney;J.;T.Tobavashi;G.H.Holm;T.S.Dermody;B.Sherry.;笛吹達史;小林剛;笛吹達史;Jennifer Zurney
• 通讯作者: Jennifer Zurney

The Reovirus mu2 Protein Inhibits Interferon Signaling Through a Novel Mechanism Involving Nuclear Accumulation of Interferon Regulatory Factor-9.

呼肠孤病毒 mu2 蛋白通过涉及干扰素调节因子 9 核积聚的新机制抑制干扰素信号传导。

• 发表时间: 2009
• 期刊: J.Vrol. 83
• 作者: Zurney;J.;T.Tobavashi;G.H.Holm;T.S.Dermody;B.Sherry.
• 通讯作者: B.Sherry.

相同組換えによって作製した新規サル指向性ヒト免疫不全ウイルスの遺伝子解析

同源重组产生的新型猿类人类免疫缺陷病毒的遗传分析

• 发表时间: 2010
• 作者: 中村仁美;大附寛幸;松田健太;小林剛;五十嵐樹彦;三浦智行
• 通讯作者: 三浦智行

Identification of Functional Domains in Reovirus Replication Proteins μNS and μ2

• DOI: 10.1128/jvi.01495-08
• 发表时间: 2009-04-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Kobayashi, Takeshi;Ooms, Laura S.;Dermody, Terence S.
• 通讯作者: Dermody, Terence S.