Examination of factors involved in transdifferentiation from mouse epidermal cells to corneal epithelial-like cells

小鼠表皮细胞向角膜上皮样细胞转分化相关因素的研究

• 批准号: 22791665
• 负责人: KOBAYASHI Takeshi
• 金额: $ 1.91万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791665/
• 关键词: 角膜上皮再生医療; 表皮前駆細胞; 角膜上皮細胞; 形質転換; ケラチン12; 角膜実質; 角膜輪部; 間葉系細胞由来因子

The corneal epithelial cells that transdifferentiated from auto-epidermal cells should be useful for the corneal regeneration. By using the epidermal cells from K12Cre/ZEG mouse, in which the GFP expression is occurred by krt12 gene expression, we determined the differentiation potential of the mouse epidermal cells into corneal epithelial-like cells on the epithelial-denuded cornea/sclera graft. The cultured tissue was examined by immunofluorescence. Our results suggested that the mouse epidermal cells could transdifferentiated into the corneal epithelial-like cells specifically on the peripheral region of cornea, where the extracellular matrix or stromal-derived soluble factors might have a key role in the induction of K12 expression or cell fate determination of the corneal epithelia.

自体表皮细胞转分化的角膜上皮细胞应该对角膜再生有用。通过使用 K12Cre/ZEG 小鼠的表皮细胞，其中 GFP 的表达是通过 krt12 基因表达来实现的，我们确定了小鼠表皮细胞在上皮剥脱的角膜/巩膜移植物上分化为角膜上皮样细胞的潜力。通过免疫荧光检查培养的组织。我们的结果表明，小鼠表皮细胞可以转分化为角膜上皮样细胞，特别是在角膜周边区域，其中细胞外基质或基质来源的可溶性因子可能在诱导 K12 表达或细胞命运决定中发挥关键作用角膜上皮细胞。

项目成果

Use of 5-cyano-2, 3-ditolyl-tetrazolium chloride(CTC) staining as Indicator of Biocidal Activity in Rapid Assay for Anti-Acanthamoeba Agents

使用 5-氰基-2, 3-二甲苯基氯化四唑 (CTC) 染色作为抗棘阿米巴药物快速测定中杀菌活性的指示剂

• 发表时间: 2012
• 期刊: J Clin Microbiol
• 影响因子: 9.4
• 作者: Kobayashi T;Mito T;Watanabe N;Suzuki T;Shiraishi A;Ohashi Y
• 通讯作者: Ohashi Y

マウス表皮細胞から形質転換した角膜上皮細胞の免疫組織学的検討

小鼠表皮细胞转化角膜上皮细胞的免疫组织学研究

• 发表时间: 2012
• 作者: 小林剛;林康人;渡辺成美;白石敦;大橋裕一
• 通讯作者: 大橋裕一

Murine Epidermal Cells can be Transdifferentiated into Corneal Epithelial-like Cells in the Corneal Epithelial Environment

小鼠表皮细胞可以在角膜上皮环境中转分化为角膜上皮样细胞

• 发表时间: 2010
• 作者: Atsushi Shiraiahi;Takeshi Kobayashi;Yasuhito Hayashi;Yuichi Ohashi
• 通讯作者: Yuichi Ohashi

Efficacy of commercial soft contact lens disinfectant solutions against Acanthamoeba

• DOI: 10.1007/s10384-011-0062-y
• 发表时间: 2011-09-01
• 期刊: JAPANESE JOURNAL OF OPHTHALMOLOGY
• 影响因子: 2.4
• 作者: Kobayashi, Takeshi;Gibbon, Lindsay;Ohashi, Yuichi
• 通讯作者: Ohashi, Yuichi

Epithelial sheets produced from cryopreserved human corneal epithelial cells using human dermal fibroblast-embedded collagen gel

使用人真皮成纤维细胞包埋的胶原凝胶从冷冻保存的人角膜上皮细胞产生上皮片

• 发表时间: 2010
• 作者: Kobayashi T;Shiraishi A;Yang L;Shirakata Y;Hashimoto K;Ohashi Y
• 通讯作者: Ohashi Y