Relationship between the common epitope and the structure of sugar chains in plant allergens

植物过敏原中共同表位与糖链结构的关系

• 批准号: 21580151
• 负责人: TSUJI Hideaki
• 金额: $ 3.16万
• 依托单位: Okayama Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580151/
• 关键词: 大豆; 小麦; アレルゲン; 糖鎖; 共通抗原; 発現タンパク質; Gly m Bd 28K; Tri a Bd 27K; 大豆アレルゲン; アレルゲン性; 糖タンパク質; 共通抗原性; 糖ペプチド; アスパラギン結合型糖鎖; 大豆; 小麦; アレルゲン; 糖鎖; 共通抗原; 発現タンパク質; Gly m Bd 28K; Tri a Bd 27K; 大豆アレルゲン; アレルゲン性; 糖タンパク質; 共通抗原性; 糖ペプチド; アスパラギン結合型糖鎖

We investigated the allergenicity of soybean allergen, Gly m Bd 28K, and wheat allergen, Tri a Bd 27K. These allergens were shown to contain fucose and xylose in addition to mannose, N-acetyl glucosamine, indicating that the sugar moieties of these allergens may be asparagine-linmked sugar chain. Furthermore, these sugar moieties were shown to be bind IgE antibodies in sera of patients sensitive to soybean and wheat.The cDNA encoding Gly m Bd 28K has been cloned. However, the cDNA has the part encoding the signal peptide of the precursor for Gly m Bd 28K, but the cDNA has not the perfect cDNA encoding full length of mRNA for the allergen. In the present study, three amino acid residues on the signal peputide and 20-bp on the 5'-untranlated region were elucidated by 5'-RACE kit. This finding provide useful information on the expression of the allergen in cultivated tabacco cells.

我们研究了大豆过敏原，Gly M BD 28K和小麦过敏原的过敏性，Tri A BD 27K。这些过敏原被证明除了甘露糖，N-乙酰葡萄糖胺外还含有岩糖和木糖，表明这些过敏原的糖部分可能是天冬酰胺 - 粘蛋白的糖链。此外，这些糖部分被证明是在对大豆和小麦敏感的患者血清中结合的IgE抗体。编码Gly M BD 28K的cDNA已被克隆。但是，cDNA具有编码Gly M BD 28K的前体的信号肽的部分，但是cDNA并不是编码过敏原的完美cDNA。在本研究中，通过5'-RACE试剂盒阐明了信号peputide上的三个氨基酸残基和5'非渗入区域的20 bp。该发现提供了有关过敏原细胞中过敏原表达的有用信息。