STUDY ON THE RELATIONSHIP BETWEEN THE STRUCTURE OF THE SUGAR MOIETIES OF PLANT ALLERGENS AND THEIR ALLERGENICITIES

植物过敏原糖基结构与其过敏性关系的研究

基本信息

批准号：
11660129
负责人：
TSUJI Hideaki
金额：
$ 2.37万
依托单位：
Okayama Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660129/
关键词：
Wheat allergen Tria Bd 17K Gly m Bd 28K Suar chain Allergenicity cDNA sugar chain allergenicity Gly m Bd28K Augar chain allergenecity

项目摘要

Nowaday, food allergy is becoming a great social problem. Allergens in food have been actively elucidated and a great number of the allergens have been found. However, as the latex-fruit syndrome, some similarity among allergens have been pointed out. As an example, the sugar moieties in plant allergens are proposed to show a common antigenicity. We observed similar phenomena cas to Gly m Bd 28K and Tri a Bd 17K.In the present study, we examined the relationship between the structure of the sugar moieties of Gly m Bd 28K and Tri a Bd 17K and their allergenicities.A cDNA encoding Gly m Bd 28K has been cloned. The product for cDNA has been shown to exhibit high homology with pumpkin MP27/MP32 and a carrot globulin-like protein. In comparison of the amino acid sequence of the product with that of the pumpkin protein, the allergen was indicated to be formed via a preproprotein from its proprotein. The allergen corresponded to the former half part of the product. Furthermore, a recombinant allergen was expressed as a fusion protein with glutathione S-transferase in Eschericia coli using an expression vector pGEX-6T.However, the recombinant allergen showed weak response against IgE antibodies in the sera of Gly m Bd 28K-sensitive patients. The glycopeptide obtained with lysyl endopeptidase from the allergen which was purified from defatted soybean flakes, showed strong response to the IgE antibodies, but the deglycosylated peptide from the glycopeptide did not react with the IgE antibodies. These findings suggest that the sugar moiety of the allergen binds to IgE antibodies in the sera of the patients, and that the sugar moiety acts as a common antigenic determinant in many plant glycoproteinic allergens.

如今，食物过敏已成为一个很大的社会问题。食物中的过敏原已积极阐明，并发现了许多过敏原。但是，作为乳胶果综合征，已经指出了过敏原之间的一些相似之处。例如，提出了植物过敏原中的糖部分以显示共同的抗原性。我们观察到与Gly M BD 28K和Tri A BD 17K相似的现象。在本研究中，我们检查了Gly M BD 28K的糖基部分的结构与Tri A BD 17K的结构与过敏性。该cDNA的产物已显示出与南瓜MP27/MP32和胡萝卜球蛋白样蛋白的高同源性。与南瓜蛋白的氨基酸序列相比，过敏原通过其前蛋白质形成过敏原。过敏原对应于产品的前一部分。此外，使用表达载体PGEX-6T表示，重组过敏原以与谷胱甘肽S-转移酶的融合蛋白表示，而重组过敏原表现出弱反应对Gly M BD 28K敏感患者的Sera Sera中IgE抗体的反应较弱。从过敏原中从纯化的大豆片纯化的过敏原中获得的糖肽对IgE抗体的反应很强，但是糖肽的退化糖基化肽与IgE抗体没有反应。这些发现表明，过敏原的糖部分与患者血清中的IgE抗体结合，并且糖部分在许多植物糖蛋白蛋白质中的常见抗原决定因素。