STUDY ON THE RELATIONSHIP BETWEEN THE STRUCTURE OF THE SUGAR MOIETIES OF PLANT ALLERGENS AND THEIR ALLERGENICITIES

植物过敏原糖基结构与其过敏性关系的研究

基本信息

批准号：
11660129
负责人：
TSUJI Hideaki
金额：
$ 2.37万
依托单位：
Okayama Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660129/
关键词：
Wheat allergen Tria Bd 17K Gly m Bd 28K Suar chain Allergenicity cDNA sugar chain allergenicity Gly m Bd28K Augar chain allergenecity

项目摘要

Nowaday, food allergy is becoming a great social problem. Allergens in food have been actively elucidated and a great number of the allergens have been found. However, as the latex-fruit syndrome, some similarity among allergens have been pointed out. As an example, the sugar moieties in plant allergens are proposed to show a common antigenicity. We observed similar phenomena cas to Gly m Bd 28K and Tri a Bd 17K.In the present study, we examined the relationship between the structure of the sugar moieties of Gly m Bd 28K and Tri a Bd 17K and their allergenicities.A cDNA encoding Gly m Bd 28K has been cloned. The product for cDNA has been shown to exhibit high homology with pumpkin MP27/MP32 and a carrot globulin-like protein. In comparison of the amino acid sequence of the product with that of the pumpkin protein, the allergen was indicated to be formed via a preproprotein from its proprotein. The allergen corresponded to the former half part of the product. Furthermore, a recombinant allergen was expressed as a fusion protein with glutathione S-transferase in Eschericia coli using an expression vector pGEX-6T.However, the recombinant allergen showed weak response against IgE antibodies in the sera of Gly m Bd 28K-sensitive patients. The glycopeptide obtained with lysyl endopeptidase from the allergen which was purified from defatted soybean flakes, showed strong response to the IgE antibodies, but the deglycosylated peptide from the glycopeptide did not react with the IgE antibodies. These findings suggest that the sugar moiety of the allergen binds to IgE antibodies in the sera of the patients, and that the sugar moiety acts as a common antigenic determinant in many plant glycoproteinic allergens.

如今，食物过敏正在成为一个巨大的社会问题。食品中的过敏原已被积极阐明，并已发现大量过敏原。然而，正如乳胶水果综合症一样，过敏原之间存在一些相似之处。例如，植物过敏原中的糖部分被认为表现出共同的抗原性。我们观察到与 Gly m Bd 28K 和 Tri a Bd 17K 类似的现象。在本研究中，我们检查了 Gly m Bd 28K 和 Tri a Bd 17K 的糖部分结构与其致敏性之间的关系。编码 Gly 的 cDNA m Bd 28K 已被克隆。 cDNA 产物已被证明与南瓜 MP27/MP32 和胡萝卜球蛋白样蛋白具有高度同源性。将产品的氨基酸序列与南瓜蛋白的氨基酸序列进行比较，表明过敏原是通过其原蛋白通过前蛋白原形成的。过敏原对应于产品的前半部分。此外，使用表达载体pGEX-6T在大肠杆菌中将重组过敏原表达为与谷胱甘肽S-转移酶的融合蛋白。然而，重组过敏原对Gly m Bd 28K敏感患者的血清中的IgE抗体表现出较弱的反应。用从脱脂大豆片中纯化的过敏原中的赖氨酰内肽酶获得的糖肽对IgE抗体表现出强烈的反应，但来自糖肽的去糖基化肽不与IgE抗体反应。这些发现表明，过敏原的糖部分与患者血清中的 IgE 抗体结合，并且糖部分在许多植物糖蛋白过敏原中充当常见的抗原决定簇。