Analysis of the role of hepatic remodeling and signal of hepatic regeneration after hepatic ischemia/reperfusion injury in obstructive jaundice

梗阻性黄疸肝缺血/再灌注损伤后肝重塑及肝再生信号的作用分析

• 批准号: 20591621
• 负责人: KATO Atsushi
• 金额: $ 3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591621/
• 关键词: 閉塞性黄疸; 阻血再灌流; 肝再生; 転写因子; 肝星細胞

Cholestasis of the liver is known to be an important risk factor for surgical morbidity and mortality after major hepatectomy. However, the mechanism of liver injury in cholestatic liver is not fully understood. Our data suggests that hepatic stellate cell activation and increased endothelin-1 production play a crucial role in hepatic ischemia/reperfusion injury in cholestatic liver. To identify predictive factors for postoperative liver regeneration after major hepatectoy, clinical factors and liver volumetric changes determined by CT volumetry and CT attenuation values (CT-AVs) were compared in patients with and without postoperative hepatic failure. Postoperative lower CT-AV was the only independent risk factor of postoperative hepatic failure. Preoperative portal vein embolization (PVE) is an effective means of creating hypertrophy of future remaining liver, which leads to safety of extensive hepatectomy for hepatic malignancies. Plasma VEGF levels were increased markedly after PVE and were maximal after 7 days of PVE. Measurement of plasma VEGF levels after PVE may be a predictive factor of potential liver regeneration and may provide useful information to decide on the schedule of subsequent hepatectomy.

已知肝脏的胆汁淤积是重大肝切除术后手术发病率和死亡率的重要危险因素。然而，尚不完全了解胆汁淤积性肝脏中肝损伤的机制。我们的数据表明，肝星状细胞激活和内皮素-1产生增加在胆汁淤积性肝脏的肝缺血/再灌注损伤中起着至关重要的作用。为了鉴定主要肝癌后术后肝脏再生的预测因素，比较了有或没有术后术后肝衰竭的患者，比较了CT体积和CT衰减值（CT-AV）确定的临床因素和肝大量变化。术后CT-AV是术后肝衰竭的唯一独立危险因素。术前门静脉栓塞（PVE）是产生未来剩余肝脏肥大的有效手段，从而导致肝脏恶性肿瘤的广泛肝切除术的安全性。 PVE后血浆VEGF水平显着升高，PVE 7天后最大。 PVE后血浆VEGF水平的测量可能是潜在肝脏再生的预测因素，并且可以提供有用的信息来决定随后的肝切除术的时间表。

项目成果

Role of Kupffer cells in tolerance induction after portal venous administration of alloantigen.

库普弗细胞在门静脉施用同种抗原后耐受诱导中的作用。

• 发表时间: 2009
• 期刊: Hepatogastroenterology 56
• 作者: Shimizu H;Kataoka M;Kimura F;Yoshidome H;Ohtsuka M;Kato A;Miyazaki M.
• 通讯作者: Miyazaki M.

One hundred seven consecutive surgical resections for hilar cholangiocarcinoma of Bismuth types II, III, IV between 2001 and 2008

2001 年至 2008 年间，连续 107 例 Bismuth II、III、IV 型肝门部胆管癌手术切除

• 发表时间: 2010
• 期刊: J Hepatobiliary Pancreat Sci 17(4)
• 作者: Miyazaki M;Kimura F;Shimizu H;Yoshidome H;Otuka M;Kato A;Yoshitomi H;Furukawa K;Takeuchi D;Takayashiki T;Suda K;Takano S.
• 通讯作者: Takano S.

Hepatocyte apoptosis is enhanced after ischemia/reperfusion in the steatotic liver.

• DOI: 10.3164/jcbn.10-74
• 发表时间: 2011-03
• 期刊: Journal of clinical biochemistry and nutrition
• 影响因子: 2.4
• 作者: Suzuki T;Yoshidome H;Kimura F;Shimizu H;Ohtsuka M;Takeuchi D;Kato A;Furukawa K;Yoshitomi H;Iida A;Dochi T;Miyazaki M
• 通讯作者: Miyazaki M

Endothelin-1 aggravates hepatic ischemia/reperfusion injury duringobstructive cholestasis in bile duct ligated mice.

Endothelin-1 会加重胆管结扎小鼠阻塞性胆汁淤积期间的肝脏缺血/再灌注损伤。

• 发表时间: 2010
• 期刊: Journal of Surgical Research
• 影响因子: 2.2
• 作者: Hiroyuki Nagai;Atsushi Kato;Fumio Kimura;Hiroaki Shimizu;Hiroyuki Yoshidome;et al.
• 通讯作者: et al.

Mechanism of neutrophil accumulation in sinusids after extrahepatic biliry obstruction.

肝外胆道梗阻后窦窦中性粒细胞积聚的机制。

• 发表时间: 2008
• 期刊: Hapetogastroenterology 55
• 作者: Wakabayashi Y;Shimizu H;Kimura F;Yoshidome H;Ohtsuka M;Kato A;Miyazaki M
• 通讯作者: Miyazaki M