Analysis of Functional Regions on The Sendai Virus Genome

仙台病毒基因组功能区分析

• 批准号: 11470077
• 负责人: KATO Atsushi
• 金额: $ 4.42万
• 依托单位: National Institute of Infections Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470077/
• 关键词: Sendai virus; paramyxovirus; reverse genetics; C protein; RNA synthesis; Interferon; Innate immunity; 終結配列; リードスルー; 粒子形成

An ORF overlapping the ammo-terminal portion of the Sendai virus (SeV) P ORF in the +1 frame produces a nested set of carboxy-coterminal proteins, C,' C, Yl and Y2, which are referred to collectively as the C proteins. The C proteins are an extremely versatile triple-role player ; they counteract the anti-viral action of interferons (IFNs), inhibit viral RNA synthesis and are involved in virus assembly. Here, we established HeLa cell lines stably expressing the C, Y1 and Y2 proteins individually and examined the capacity of these cells to circumvent the anti-viral action of IFNs and to inhibit the viral transcription. The data obtained clearly indicated that the smallest Y2 protein was as active as the C and Y1 proteins in both counteracting the anti-viral action of IFNs and inhibiting viral transcription.

+1框架中仙台病毒（SEV）p ORF的弹药末端部分重叠的ORF产生一组嵌套的羧基蛋白质，C，'C，YL和Y2，被称为C蛋白。 C蛋白是非常通用的三杆播放器。它们抵消了干扰素（IFN）的抗病毒作用，抑制病毒RNA合成并参与病毒组装。在这里，我们建立了稳定表达C，Y1和Y2蛋白的HeLa细胞系，并检查了这些细胞绕过IFN的抗病毒作用并抑制病毒转录的能力。获得的数据清楚地表明，最小的Y2蛋白在抵消IFN的抗病毒作用和抑制病毒转录的抗病毒作用时与C和Y1蛋白一样活跃。

项目成果

A.Kato他: "Sendai virus gene start signals are not equivalent in reinitiation capacity : Moderation at the fusion protein gene"Journal of Virology. 73. 9237-9246 (1999)

A. Kato 等人：“仙台病毒基因起始信号的重新启动能力不同：融合蛋白基因的调节”Journal of Virology 73. 9237-9246 (1999)。

H.A. Koyama, M. Ogawa, A. Kato, Y. Nagai, and A. Adachi: "Lack of apoptosis in Sendai virus-infected Hep-2 cells without participation of viral antiapoptosis gene"Microbes Infect. 3. 1115-1121 (2001)

哈。

A. Kato, Y. Ohnishi, M. Kohase, S. Saito, M. Tashiro and Y. Nagai: "Y2, smallest of the Sendai virus C protein, is fully capable of both counteracting the antiviral action of interferons and inhibiting viral RNA synthesis"J. Virol. 75. 3802-3810 (2001)

A. Kato、Y. Ohnishi、M. Kohase、S. Saito、M. Tashiro 和 Y. Nagai：“Y2 是仙台病毒 C 蛋白中最小的一种，完全能够抵消干扰素的抗病毒作用并抑制病毒 RNA

A.Kato 他: "Y2, smallest of the Sendai virus C protein, is fully capable of both counteracting the antiviral action of interferons and inhibiting viral RNA synthesis"J. Virol.. 75. 3802-3810 (2001)

A. Kato 等人：“Y2 是仙台病毒 C 蛋白中最小的一种，完全能够抵消干扰素的抗病毒作用并抑制病毒 RNA 合成” J. Virol.. 75. 3802-3810 (2001)

T.Sakaguchi他: "Double-layered membrane vesicles released from mammalian cells infected with Sendai virus expressing the matrix protein of vesicular stomatitis virus"Virology. 263. 230-243 (1999)

T. Sakaguchi 等人：“感染仙台病毒的哺乳动物细胞释放的双层膜囊泡，表达水泡性口炎病毒的基质蛋白”，《病毒学》263. 230-243 (1999)。