Immunological prevention of type 1 diabetes using GAD65-encording DNA vaccine

使用编码 GAD65 的 DNA 疫苗对 1 型糖尿病进行免疫预防

• 批准号: 20591056
• 负责人: KOBAYASHI Tetsuro
• 金额: $ 3万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591056/
• 关键词: GAD65; 1型糖尿病; 膵島炎; GAD抗体; アミラーゼ抗体; GAD; β-細胞; エピトープ

Preventive effect of human glutamic acid decarboxylase (GAD) 65 (GAD65) vaccination for type 1 diabetes was examined using NOD mice model. Subcutaneous electropolation of site-specific GAD65 fragments including GAD65 (1-83aa : N-GAD65), GAD65 (244-443aa : Center-GAD65) and GAD65 (443-585aa : C-GAD65) were done to 4 week female NOD mice.Specific T cell responses to N-GAD65, Center-GAD65 and C-GAD65 were observed to respective GAD65 fragments in electropolated NOD mice. However, occurrence rate of diabetes in GAD65 vector- or empty vector- electropolated groups did not reach significant levels during 30 weeks.Human GAD65 different from mice GAD65 may explain a failure to prevent the occurrence of diabetes by human site-specific GAD65 vaccination.

使用 NOD 小鼠模型检查人谷氨酸脱羧酶 (GAD) 65 (GAD65) 疫苗接种对 1 型糖尿病的预防效果。对 4 周雌性 NOD 小鼠进行位点特异性 GAD65 片段（包括 GAD65（1-83aa：N-GAD65）、GAD65（244-443aa：Center-GAD65）和 GAD65（443-585aa：C-GAD65））的皮下电插。对 N-GAD65、Center-GAD65 和的特异性 T 细胞反应在电插NOD小鼠中观察到C-GAD65各自的GAD65片段。然而，GAD65载体或空载体电转组中的糖尿病发生率在30周期间并未达到显着水平。人GAD65与小鼠GAD65的不同可能解释了通过人位点特异性GAD65疫苗接种未能预防糖尿病发生的原因。

项目成果

Pathlogical changes in the pancreas of fulminant type 1 diabetes and slowly progressive insulin-dependent(type 1)diabetes

暴发性1型糖尿病和缓慢进展型胰岛素依赖型（1型）糖尿病的胰腺病理改变

• 发表时间: 2010
• 作者: Ando H;Ushijima K;Kumazaki M;Takamura T;Yokota N;Saito T;Irie S;Kaneko S;Fujimura A;T.Kobayashi
• 通讯作者: T.Kobayashi

UK-Japan Workshop ~Frontiers of Diabetes Research~ ; Causes, Pathophysiology and Treatment of Type 1 Diabetes in Japan

英国-日本研讨会~糖尿病研究前沿~；

• 发表时间: 2009
• 作者: Nishimura S;Manabe I;Naga;Eto K;Yamashita H;Ohsugi M;Otsu M;Hara K;Sugiura S;Yoshimura K;Kadowaki T;Nagai R;Tetsuro Kobayashi
• 通讯作者: Tetsuro Kobayashi

A novel autoantibody against heat shock protein 10 in diabetes associated with autoimmune pancreatitis and fulminant type 1 diabetes.

一种针对与自身免疫性胰腺炎和暴发性 1 型糖尿病相关的糖尿病中的热休克蛋白 10 的新型自身抗体。

• 发表时间: 2008
• 作者: Souichi Takizawa;Toyoshi Endo;Shoichiro Tanaka;Masashi Takahashi;Tetsuro Kobayashi
• 通讯作者: Tetsuro Kobayashi

on behalf of the Committee on Type 1 Diabetes, Japan Diabetes Society. Differential association of HLA with three subtypes of type 1 diabetes : fulminant, slowly progressive and acute-onset.

代表日本糖尿病学会 1 型糖尿病委员会。

• 发表时间: 2009
• 期刊: Diabetologia 52
• 作者: Y. Kawabata;H. Ikegami;T.Awata;A.Imagawa;T.Maruyama;E.Kawasaki;S.Tanaka;A.Shimada;H.Osawa;T.Kobayashi;T.Hanafusa;K.Tokunaga;H.Makino
• 通讯作者: H.Makino

Immunization with thyroglobulin induces Graves'-like disease in mice.

• DOI: 10.1677/joe-09-0089
• 发表时间: 2009-08
• 期刊: The Journal of endocrinology
• 作者: Endo T;Kobayashi T
• 通讯作者: Kobayashi T