Real time and in situ observation of actin structure in migrating leukocyte

迁移白细胞肌动蛋白结构的实时原位观察

• 批准号: 20500402
• 负责人: KATAOKA Noriyuki
• 金额: $ 2.83万
• 依托单位: Kawasaki University of Medical Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500402/
• 关键词: 白血球; 内皮細胞; 情報伝達; ナノバイオロジー・ナノメディスン; 動脈硬化; アクチンフィラメト; ナノバイオロジー; ナノメディスン

It is well known that one of the critical events in early atherosclerosis is the recruitment of blood monocytes to proatherogenic vascular regions and subsequent transmigration across vascular endothelial cells. It is still unknown how monocytes infiltrate into between and underneath EC monolayer. To elucidate this phenomenon, direct observation of cytoskeletal structure and/or signaling molecules in migrating monocytes under the controlled culture condition is necessary. In this study, we have established the observation system of migration of monocytic cell line induced by chemoattractant from micropipette. We have estimated the migration of HL-60 and THP-1 seeded on gelatin or fibronectin, to fMLP or MCP-1. In our experiments, THP-1 cells seeded on fibronectin actively migrated to the tip of micropipette. fMLP was more effective than MCP-1. In addition, we have found that PECAM-1 in EC is one of the key molecules that controls the leukocytes trans-endothelial migration and SHP-2 exists in the upstream of signaling transduction.

众所周知，早期动脉粥样硬化中的关键事件之一是将血单核细胞募集到促进性血管区域以及随后跨血管内皮细胞的转移。尚不清楚单核细胞如何浸润到EC单层之间和下方。为了阐明这种现象，必须直接观察在受控培养条件下迁移单核细胞中细胞骨架结构和/或信号分子。在这项研究中，我们建立了由趋化剂从微孔引起的单核细胞系的迁移系统。我们已经估计，HL-60和THP-1在明胶或纤连蛋白上播种到FMLP或MCP-1的迁移。在我们的实验中，在纤连蛋白上播种的Thp-1细胞积极迁移到微孔尖端。 FMLP比MCP-1更有效。此外，我们发现EC中的PECAM-1是控制白细胞跨内皮迁移的关键分子之一，而SHP-2则存在于信号转导的上游。

项目成果

An Experimental Model for Studying Molecular Behavior of Platelet-Endothelial Cell Adhesion Molecule-i during Mechanical Interactions between Monocytes and Vascular Endothelial Cells

研究单核细胞与血管内皮细胞机械相互作用过程中血小板-内皮细胞粘附分子-i分子行为的实验模型

• 发表时间: 2010
• 期刊: Journal of Biomechanical Science and Engineering
• 作者: J. P. Owen;D. P. Wipf;H. T. Attias;K. Sekihara;S. S. Nagarajan;Ken Hashimoto
• 通讯作者: Ken Hashimoto

単球の内皮下浸潤によって誘発される次の単球浸潤の促進

促进单核细胞内皮下浸润诱导的后续单核细胞浸润

• 发表时间: 2010
• 作者: Matsui K;Matsui A;Handa T;Kawai T;Suzuki O;Kamakura S;Echigo S.;脇和規,他;橋本謙
• 通讯作者: 橋本謙

Role of dynamic recruitment of Endothelial PECAM-1 to Transmigrating Monocytes

内皮 PECAM-1 动态募集到迁移单核细胞的作用

• 发表时间: 2009
• 作者: Jin Nakamura;Akiko Obata;Toshihiro Kasuga;Noriyuki Kataoka
• 通讯作者: Noriyuki Kataoka

Local Dynamic Recruitment of Endothelial PECAM-1 to Transmigrating Monocytes

内皮 PECAM-1 向迁移单核细胞的局部动态募集

• 发表时间: 2008
• 作者: K. Kawakami;et. al.;Kataoka N.
• 通讯作者: Kataoka N.

Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension

• DOI: 10.1016/j.ijcard.2008.11.022
• 发表时间: 2010-04-01
• 期刊: INTERNATIONAL JOURNAL OF CARDIOLOGY
• 影响因子: 3.5
• 作者: Nakamura, Kazufumi;Shimizu, Juichiro;Ohe, Tohru
• 通讯作者: Ohe, Tohru