Biomechanics of Leukocytes migration on Endothelial Cell Monolayer

白细胞在内皮细胞单层上迁移的生物力学

• 批准号: 23500535
• 负责人: KATAOKA Noriyuki
• 金额: $ 3.41万
• 依托单位: Kawasaki University of Medical Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500535/
• 关键词: 血管内皮細胞; 単球; アクチンフィラメント; アテローム性動脈硬化; 内皮細胞; 単球浸潤; 細胞メカニクス; 細胞工学; 動脈硬化

It is well known that one of the critical events in early atherosclerosis is the recruitment of blood monocytes to proatherogenic vascular regions and subsequent transmigration across vascular endothelial cells. To elucidate this phenomenon, we have observed the effects of cytoskeletal structure. Human umbilical vein endothelial cells (Huvecs) were cultured on the porous membrane and/or cell culture dishes. Huvecs were stimulated with IL-1beta and oxLDL. Then monocytes isolated from human blood were added to Huvec monolayer, counted migrated monocytes underneath Huvecs and observed monocytes behavior. When Huvecs were treated with cytochalasin D which blocks polymerization and the elongation of actin, migrated monocytes were decreased. That means that actin filaments are important cytosolic structure for monocytes trans-endothelial migration. In addition, Ellagic Acid hydrolyzed from Ellagitannin which is bioactive polyphenols from pomegranate, has been shown anti-atherogenic effect.

众所周知，早期动脉粥样硬化中的关键事件之一是将血单核细胞募集到促进性血管区域以及随后跨血管内皮细胞的转移。为了阐明这种现象，我们观察到了细胞骨架结构的影响。人脐静脉内皮细胞（HUVEC）在多孔膜和/或细胞培养皿上培养。用IL-1BETA和OXLDL刺激HUVEC。然后将从人血中分离的单核细胞添加到HUVEC单层，在HUVEC下方计数的迁移单核细胞，并观察到单核细胞行为。当HUVEC用细胞切拉蛋白D处理，阻断聚合和肌动蛋白的伸长时，迁移的单核细胞降低。这意味着肌动蛋白丝是单核细胞跨内皮迁移的重要胞质结构。此外，已经显示出从石榴酸盐的生物活性多酚水解的椭圆酸已显示出抗动脉粥样硬化作用。

项目成果

Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic β-Adrenergic Stimulation

慢性β-肾上腺素能刺激引起的肥大大鼠心脏横向被动硬度增加以及残余肌动蛋白和肌球蛋白跨桥形成

• DOI: 10.1007/s12576-011-0181-8
• 发表时间: 2012
• 期刊: Circulation Journal
• 影响因子: 3.3
• 作者: W.S. Yoshikawa;K. Nakamura;D. Miura;J. Shimizu;K. Hashimoto;N. Kataoka;et al.
• 通讯作者: et al.

Realtime observation of actindynamics in living and migrating monocytic cells with Lifeact-GFP fusion protein

使用 Lifeact-GFP 融合蛋白实时观察活体和迁移单核细胞的肌动动力学

• 发表时间: 2012
• 作者: N. Kataoka;K. Hashimoto;T. Okamoto;S. Mohri;F. Kajiya
• 通讯作者: F. Kajiya

Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic beta-Adrenergic Stimulation

慢性β-肾上腺素能刺激引起的肥大大鼠心脏横向心肌细胞被动硬度增加以及残余肌动蛋白和肌球蛋白跨桥形成

• 发表时间: 2013
• 期刊: Circulation Journal
• 影响因子: 3.3
• 作者: Sumita Yoshikawa W;Nakamura K;Miura D;Shimizu J;Hashimoto K;Kataoka N;Toyota H;Okuyama H;Miyoshi T;Morita H;Fukushima Kusano K;Matsuo T;Takaki M;Kajiya F;Yagi N;Ohe T;Ito H
• 通讯作者: Ito H

アクチン結合ペプチドLifeact を用いた遊走白血球中のアクチンダイナミクス観察

使用肌动蛋白结合肽 Lifeact 观察白细胞迁移中的肌动蛋白动态

• 发表时间: 2013
• 作者: 片岡則之;橋本謙;坂元尚哉;岡本威明;毛利聡
• 通讯作者: 毛利聡

Live-cell visualization of the trans-cellular mode of monocyte transmigration across the vascular, endothelium, and its relationship with endothelial PECAM-1

单核细胞跨血管、内皮细胞迁移的跨细胞模式的活细胞可视化及其与内皮 PECAM-1 的关系

• 发表时间: 2012
• 期刊: The Journal of Physiological Sciences
• 作者: K. Hashimoto;N. Kataoka;E. Nakamura;K. Hagihara;T. Okamoto;H. Kanouchi;S. Mohri;K. Tsujioka;F. Kajiya
• 通讯作者: F. Kajiya