Study on endocrine disruptors of dental materials using an amphibian system

利用两栖系统研究牙科材料的内分泌干扰物

• 批准号: 19592239
• 负责人: NOMURA Yuji
• 金额: $ 2.83万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592239/
• 关键词: 生体材料; 環境ホルモン; 両生類; 歯学; 安全性評価

Risk assessment of EDCs have gradually been clarified by the screening test that used a yeast and bacteria such as competitive estrogen receptor-binding assay and reporter assay of genes containing estrogen-responsive promoters about these chemical substance. However, bioassay systems are also needed to elucidate direct effects of the compounds on animals and humans because environmental compounds may be metabolically transformed into estrogenic or non-estrogenic substances in the body or cells. Therefore, the screening test of estrogen and thyroid hormone activities is needed and we developed the new system using an amphibian system. By this system we evaluated the estrogen and thyroid hormone activities of dental materials. As the result, some of dental materials indicated the estrogen and thyroid hormone activities.

通过筛查测试逐渐阐明了EDC的风险评估，该测试使用了酵母和细菌，例如竞争性雌激素受体结合测定法和对这些化学物质的含有雌激素反应启动子的基因的记者测定。但是，还需要生物测定系统来阐明化合物对动物和人类的直接影响，因为可以将环境化合物代谢转化为体内或细胞中的雌激素或非雌激素物质。因此，需要对雌激素和甲状腺激素活性进行筛查测试，我们使用两栖动物系统开发了新系统。通过该系统，我们评估了牙科材料的雌激素和甲状腺激素活性。结果，一些牙科材料表明雌激素和甲状腺激素活性。

项目成果

High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma

• DOI: 10.1007/s00428-006-0327-x
• 发表时间: 2007-01-01
• 期刊: VIRCHOWS ARCHIV
• 影响因子: 3.5
• 作者: Bhawal, Ujjal Kumar;Sugiyama, Masaru;Kuniyasu, Hiroki
• 通讯作者: Kuniyasu, Hiroki