AN EXPERIMENTAL STUDY OF MACROENCAPSULATED ARTIFICIAL PANCREAS USING HOLLOW FIBER DERIVED FROM POLYSULFONE MEMBRANE

使用聚砜膜中空纤维微囊化人工胰腺的实验研究

• 批准号: 10671100
• 负责人: NOMURA Yuji
• 金额: $ 2.05万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671100/
• 关键词: Islet transplantation; Artificial pancreas; 人工膵臓

The yield and function of rat pancreatic islets were investigated, using Liberase for isolation. We used polysulfone hollow fiber as macroencapsulation materials, which the inner diameter was 0.5 mm, the outer one was 0.8 mm, and the length was 60 cm.We used a gradient separation technique newly developed by Field et al., in which albumin-coated (35%BSA) rat islets were isolated in Dextran. The islets yield were 875±746 per pancreas. The viability of islets were investigated by perifusion which an artificial pancreas was incubated in RPMI solution containing a glucose concentration of 60mg/dl for 30 minutes followed by a 30 minute period of glucose at 300 mg/dl followed by a final period of glucose at 60 mg/dl.Macroencapsulated islets responded well to glucose stimulation.Polysulfone hollow fiber, which is biocompatible since deriving from dyalyzer, is worth examining the possibility as an membrane for macroencapsulation. Further investigation will be necessary.

使用liberase进行隔离研究了大鼠胰岛的产量和功能。我们使用多硫酮中空纤维作为宏观塑料材料，内径为0.5毫米，外部直径为0.8 mm，长度为60 cm。我们使用了Field等人新开发的梯度分离技术，在该技术中，在Dextran中分离了专辑涂料（35％BSA）大鼠小鼠。胰岛产量为每胰腺875±746。 The viability of islets were investigated by period which an artificial pancreas was incubated in RPMI solution containing a glucose concentration of 60mg/dl for 30 minutes followed by a 30 minute period of glucose at 300mg/dl followed by a final period of glucose at 60mg/dl.Macroencapsulated islets responded well to glucose stimulation.Polysulfone hollow fiber, which is自从源自Dyalyzer以来，具有生物相容性，值得研究作为膜囊泡的膜的可能性。需要进一步调查。

项目成果

Nomura Y., Tomikawa S., Beck Y.: "Basic investigation of macroencapsulated artificial Pancreas. -Limitation of unpuritied islet transplantation-"Abstract book of 7 th World Congress of the International Pancreas & Islet Transplant Association. 166 (1999)

Nomura Y.、Tomikawa S.、Beck Y.：“大封装人工胰腺的基础研究。-未纯化胰岛移植的限制-”第七届世界胰腺大会摘要书

Nomura Y.: "Basic investigation of macroencapsulated artificial pancreas"Abstract book of 7th World Congress of the International Pancreas & Islet transplant Association. 166-166 (1999)

野村Y.：《大封装人工胰腺的基础研究》第七届世界胰腺大会摘要书

Y.Nomura,et al.: "Basic investigation of macro encapsulated artificial pancreas. -Limitation of unpurified islet transplantation-"Abstract book of 7th World Congress of the International Pancreas & Islet Transplant Association. 166 (1999)

Y.Nomura等：“大封装人工胰腺的基础研究。-未纯化胰岛移植的局限性-”第七届世界胰腺大会摘要书