Process of osteointegration of heat-treated bone graft

热处理骨移植物的骨整合过程

基本信息

批准号：
08671651
负责人：
SATO Keiji
金额：
$ 1.47万
依托单位：
Aichi Medical University (1997-1998)Nagoya University (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

In vivo experiment: Periodical maging modalities such as MRI and Radiogram could not reveal any differences in osteointegrating process between 70℃-treated bone graft and fresh bone graft, especially bone union and marrow changes. Histology disclosed that degree of vascular invasion and osteoblast invasion were less obvious in heat-treated bone graft, Sample of fresh bone graft obviously decreased in its mineral content than that of heat-treated bone graft with significant difference since 2 months after grafting. Compression strength of the sample of fresh bone graft gradually decreased after grafting and reached bottom around 6 month, and recovered to normal strength until 1 year after grafting. On the other hand, that of heat-treated bone had kept its strength until 1 year after grafting and then reached lowest level around 1 year and half. After that point, strength of heat-treated bone recovered until 2 years, This means remodeling of the heat-treated bone is more slow than that o … More f fresh bone graft, In vitro experiment: Quantification of type 1 collagen and osteocalcin in culture medium at 3, 5 and 7th day of culture, produced by osteoblast like cells cultured on the pulverized bone powder of 70℃-treated bone, 120℃-treated bone and fresh bone were done. Quantity of type 1 collagen and osteocalcin produced by cells cultured on l20℃treated bone were markedly increased than that of cells on 70℃-treated bone and fresh bone (osteocalcin p<0.0001, type 1 collagen p<0.0002) with significant differences. Osteocalcin level produced by cells cultured on the l20℃-treated bone decreased gradually. On the other hand, type 1 collagen level increased This means osteoblast, like cells cultured on unfamiliar atmosphere such as l20℃-treated bone were forced to be differentiated and high level of osteocalcin and type 1 collagen should be produced. However, 70℃-treated bone and fresh bone provided natural condition to osteoblast-like cells and these cells could grow with low level of production of osteocalcin and type 1 collagen. Less

体内实验：MRI和X线等定期影像学检查无法显示70℃处理的骨移植物与新鲜骨移植物的骨整合过程存在任何差异，尤其是骨愈合和骨髓变化，显示血管侵袭和成骨细胞侵袭程度不同。热处理骨移植样品的矿物质含量较热处理骨移植样品明显降低，且从移植后2个月开始，新鲜骨移植样品的抗压强度逐渐降低，差异显着。移植后6个月左右达到最低水平，直到移植后1年才恢复到正常强度；而热处理骨的强度直到移植后1年才保持其强度，然后在1年左右和之后达到最低水平。到那时，热处理骨的强度直到 2 年后才恢复，这意味着热处理骨的重塑比新鲜骨移植物更慢，体外实验：培养物中 1 型半胶原和骨钙蛋白的定量中等在培养第3、5和7天，测定在70℃处理骨、120℃处理骨和新鲜骨的粉碎骨粉上培养的成骨细胞样细胞产生的1型胶原和骨钙素的量。 l20℃处理的骨比70℃处理的骨和新鲜骨上的细胞显着增加（骨钙素p<0.0001，1型胶原p<0.0002），在l20℃处理的骨骼上培养的细胞产生的骨钙素水平逐渐下降，另一方面，1型胶原水平增加，这意味着成骨细胞，就像在陌生的气氛（例如l20℃处理）中培养的细胞一样。骨骼被迫分化，应该产生高水平的骨钙素和1型胶原蛋白，但是70℃处理的骨骼和新鲜骨骼为成骨细胞样细胞提供了自然条件，这些细胞可以生长。骨钙素和 1 型胶原蛋白的产生量较少。