Isolation of human motile sperm without chromosomal error, and induction of acrosome reacti

无染色体错误的人活动精子的分离及顶体反应的诱导

• 批准号: 08457444
• 负责人: KANEKO Satoru
• 金额: $ 2.3万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457444/
• 关键词: human sperm; acrosome reaction; chronosome; motility; chromosonal error; 生体反応; Cr^+; カフェイン; アセチルコリン

Caffeine (Caf) Stimulates both sperm motility and acrosome reaction (AR), and the action is considered to be appeared through inhibition of phosphodiesterase and resulting accumulation of cAMP.The present study examined the effect of calcium channel blocker on AR,and concluded that the action mechanisms of Caf on motillity and AR were different each other. Motility was stimulated by cyclic AMP,in contrast the action of Caf on AR was appeared through the stimulation of calcium influx, but not cAMP accumulation.The present study discovered the presence of adenosine in human seminal plasma. Adenosine receptor agoist, PIA,stimulated AR and the antagosist, 8-SPT,suppressed AR.This facts suggested the posibility that sperm surface adenosine receptor contribute to the induction of AR.The sperm can not induce AR in the seminal plasma, and the abstinence form the seminal plasama is the trigger of spontaneous induction of AR.The present results proposed the working hypothesis that adenosine in the seminal plasma was the repressing factor in the seminal plsama, and the action of Caf was appeared through as the antagonist against the adenosine receptor.

咖啡因 (Caf) 刺激精子活力和顶体反应 (AR)，该作用被认为是通过抑制磷酸二酯酶和由此产生的 cAMP 积累而出现的。本研究检查了钙通道阻滞剂对 AR 的影响，并得出结论： Caf对运动性和AR的作用机制彼此不同。环AMP刺激运动，而Caf对AR的作用是通过刺激钙内流而不是cAMP积累而出现的。本研究发现人精浆中存在腺苷。腺苷受体激动剂PIA刺激AR，拮抗剂8-SPT抑制AR。这一事实表明精子表面腺苷受体可能有助于AR的诱导。精子不能诱导精浆中的AR，并且禁欲精浆中的腺苷是 AR 自发诱导的触发因素。目前的结果提出了这样的工作假设：精浆中的腺苷是精浆中的抑制因子。 Caf 的作用是通过作为腺苷受体的拮抗剂而显现的。

项目成果

S Kaneko,A Nozawa,Y Kuroda,T Oda,K Takeuchi,Y Yoshimura: "Simultaneous determination of human sperm adenyl purine nucleotides and nucleoside by means of high performance liquid chromatography-prelabel fluorescent detection system" Advamce in Reprod. 1. 29

S Kaneko，A Nozawa，Y Kuroda，T Oda，K Takeuchi，Y Yoshimura：“通过高效液相色谱-预标记荧光检测系统同时测定人精子腺嘌呤核苷酸和核苷”Advamce in Reprod。

Y Kuroda, S Kaneko, Ta Oda, Y Yoshimura, S Nozawa: "Quantitative assessment of human sperm acrosome reaction by using fluorescein isothiocyanate conjugated Concanavalin A" Arch Androl. 40. 215-224 (1998)

Y Kuroda、S Kaneko、Ta Oda、Y Yoshimura、S Nozawa：“使用异硫氰酸荧光素缀合伴刀豆球蛋白 A 定量评估人类精子顶体反应”Arch Androl。

Y Kuroda,S Kaneko,Ta Oda,Y Yoshimura,S Nozawa: "Quantitative assessment of human sperm acrosome reaction by using fluorescein isothiocyanate conjugated Concanavalin A" Arch Androl. 40. 215-224 (1998)

Y Kuroda、S Kaneko、Ta Oda、Y Yoshimura、S Nozawa：“使用异硫氰酸荧光素缀合伴刀豆球蛋白 A 定量评估人类精子顶体反应”Arch Androl。

Y Kuroda, S Kaneko, Ta Oda, Y Yoshimura, S Nozawa: "Quantitative assessment of human sperm acrosome reaction by using fluorescein isothiocyanate conjugated Concanavalin A" Arch Androl. 40. 215-224 (1998)

Y Kuroda、S Kaneko、Ta Oda、Y Yoshimura、S Nozawa：“使用异硫氰酸荧光素缀合伴刀豆球蛋白 A 定量评估人类精子顶体反应”Arch Androl。

S Kaneko, A Nozawa, Y Kuroda, T Oda, K Takeuchi, Y Yoshimura: "Simultaneous determination of human sperm adenyl purine nucleotides and nucleoside by means of high performance liquid chromatography-prelabel fluorescent detection system" Advamce in Reprod.

S Kaneko、A Nozawa、Y Kuroda、T Oda、K Takeuchi、Y Yoshimura：“通过高效液相色谱-预标记荧光检测系统同时测定人精子腺嘌呤核苷酸和核苷”Advamce in Reprod。