Enhanced safety of infertile therapy by means of quality control of sperm - relationship between vacuole in sperm head and DNA fragmentation

通过精子质量控制提高不孕症治疗的安全性——精子头部液泡与DNA碎片之间的关系

• 批准号: 22591837
• 负责人: KANEKO Satoru
• 金额: $ 3万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591837/
• 关键词: 品質管理; 精子頭部空胞; DNA 損傷; 顕微授精; 染色体; DNA損傷; ヒト精子; DNA; 電気泳動; DNA断片化; DNA fiber

The early stage of DNA fragmentation in a single nucleus could be observed by single cell pulse field gel electrophoresis (SCPFGE). First, a few large fibrous fragments were derived from a bundle of long chain fibers, the cleavages were advanced until finally almost all the DNA was shredded to granular fragments. There found no significant correlation between DNA fragmentation and vacuole in sperm head. The cell culture under high oxygen concentration caused increment of the percentage of DNA fragmentation.

单细胞脉冲场凝胶电泳（SCPFGE）可以观察到单核DNA片段化的早期阶段。首先，从一束长链纤维中衍生出一些大的纤维片段，继续进行裂解，直到最后几乎所有的DNA都被撕碎成颗粒状片段。未发现DNA碎片与精子头部液泡之间存在显着相关性。高氧浓度下的细胞培养导致DNA断裂百分比增加。

项目成果

Cell Handling and Culture Under Controlled Oxygen Concentration, in "Biomedical Tissue Culture"

“生物医学组织培养”中的受控氧气浓度下的细胞处理和培养

• 发表时间: 2012
• 作者: Satoru Kaneko; Kiyoshi Takamatsu
• 通讯作者: Kiyoshi Takamatsu

Cell Handling and Culture Under Controlled Oxygen Concentration

受控氧气浓度下的细胞处理和培养

• 发表时间: 2012
• 作者: Satoru Kaneko; Kiyoshi Takamatsu
• 通讯作者: Kiyoshi Takamatsu

Single- Cell Pulsed-Field Gel Electrophoresis toDetect the Early Stage of DNA Fragmentation in Human Sperm Nuclei

单细胞脉冲场凝胶电泳检测人类精子细胞核 DNA 碎片的早期阶段

• DOI: 10.1371/journal.pone.0042257
• 发表时间: 2012
• 影响因子: 3.7
• 作者: Kaneko S;Yoshida J;Ishikawa H;Takamatsu K
• 通讯作者: Takamatsu K